Microbiology Labs II
- Page ID
- 3190
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)- 2: Aseptic Technique and Transfer of Microorganisms
- 2.1: Introduction
- 2.2: Aseptic Technique
- 2.3: Using a bacto-Incinerator to Sterilize the Inoculating Loop
- 2.4: Transferring the Inoculum into a Broth Tube
- 2.5: Transferring the Inoculum into a Petri Plate
- 2.6: Forms of Culture Media/Oxygen and Temperature Requirements
- 2.7: Colony Morphology and Pigmentation
- 2.8: Procedure
- 2.9: Results
- 2.10: Performance Objectives for Lab 2
- 2.11: Self Quiz
- 2.12: Concept Map
- 7: Endospore Stain and Bacterial Motility
- 7.1: Introduction
- 7.2: Examples of Endospores
- 7.3: Endspore Procedures and Results
- 7.4: Bacterial Motility
- 7.5: Examples of Bacterial Motility
- 7.6: Observation of Bacterial Motility
- 7.7: Testing for Bacterial Motility
- 7.8: Procedures
- 7.9: Results
- 7.10: Perfomance Objectives for Lab 7
- 7.11: Self Quiz
- 7.12: Concept Map
- 8: Using Biochemical Testing to Identify Bacteria
- 8.1: Introduction
- 8.2: Starch Hydrolysis w. Procedures and Results
- 8.3: Protein Hydrolysis w. Procedures and Results
- 8.4: Fermentation of Carbohydrates w. Procedures and Results
- 8.5: Indole and Hydrogen Sulfie Production w. Procedures and Results
- 8.6: Catalase Activity (Demonstration)
- 8.7: Performance Objectives for Lab 8
- 8.8: Self Quiz
- 8.9: Concept Map
- 12: Isolation and Identification of Enterobacteriaceae and Pseudomonas, Part 1
- Labs 12 and 13 deal with opportunistic and pathogenic fermentative Gram-negative bacilli that are members of the bacterial family Enterobactereaceae, as well as nonfermentative Gram-negative bacilli such as Pseudomonas and Acinetobacter.
- 12.1: Introduction
- 12.2: Enterobacteriaceae - glucose fermenting, Gram-negative, enteric bacilli
- 12.3: Pseudomonas - glucose non-ermenting, Gram-negative, enteric bacilli
- 12.4: Scenerios for this Lab
- 12.5: Procedures
- 12.6: Results from Testing Procedures Used in This Lab
- 12.7: Performance Objectives for Lab 12
- 12.8: Self Quiz
- 12.9: Concept Map
- 14: Gram-Positive Streptococci- Isolation and Identification of Streptococci and Enterococci
- 14.1: Introduction
- 14.2: The genus Streptococcus
- 14.3: The Beta Streptococci
- 14.4: The Pneumococcus (Streptococcus pneumoniae)
- 14.5: Isolation an Identifiation of Pneumococci
- 14.6: Isolation an Identifiation of Enterococci
- 14.7: Case Studies
- 14.8: Procedures for Case Study #1
- 14.9: Results for Case Study #1
- 14.10: Procedures for Case Study #2
- 14.11: Results for Case Study #2
- 14.12: Performance Objectives for Case Study #2
- 14.13: Self Quiz
- 14.14: Concept Map
- 15: Gram-Positive Streptococci- Isolation and Identification of Staphylococci
- The neisseriae are a group of Gram-negative diplococci 0.6-1.5 µm in diameter (see Fig. 1A). Two species of Neisseria, N. gonorrhoeae and N. meningitidis, are considered as true human pathogens. Both of these organisms possess pili and adhesins for adherence to host cells, produce endotoxins, and resist destruction within phagocytes. N. meningitidis also produces a capsule to resist phagocytic engulfment.
- 16: Serology, Direct and Indirect Serologic Testing
- 16.1: Introduction to Serologic Testing
- 16.2: Direct Serologic Testing- Using Antigen-Antibody Reactions in the Laboratory to Identify Unknown Antigens Such as Microorganisms
- 16.3: Indirect Serologic Testing- Using Antigen-Antibody Reactions in the Laboratory to Indirectly Diagnose Disease
- 16.4: Sensitivity and Specificity of Serologic Testing
- 16.5: Procedure for Direct Serologic Testing
- 16.6: Results for Direct Serologic Testing
- 16.7: Procedure for Indirect Serologic Testing
- 16.8: Results for Indirect Serologic Testing
- 16.9: Performance Objectives for Lab 16
- 16.10: Self Quiz
- 16.11: Concept Map
- 18: Use of Chemical Agents to Control of Microorganisms
- 18.1: Disinfectants, Antiseptics, and Sanitizers
- 18.2: Evaluation of Disinfectants, Antiseptics, and Sanitizers
- 18.3: Effectiveness of Hand Washing
- 18.4: Antimicrobial Agents
- 18.5: Microbial Resistance
- 18.6: Susceptibility Testing
- 18.7: Procedure and Results
- 18.8: Performance Objectives for Lab 18
- 18.9: Self Quiz
- 18.10: Concept Map
- 21: Appendicies
- 21.1: Appendix A - Colony Morphology on Agar Plate Cultures
- 21.2: Appendix B - Scientific Notation and Dilutions
- 21.3: Appendix C - The Complete Blood Count (CBC)
- 21.4: Appendix D - Urinalysis (Dipstick Test)
- 21.5: Appendix E - Bacterial Examination of Water- Coliform Counts
- 21.6: Appendix F - SIRS AND SEPSIS