2.10: Performance Objectives for Lab 2

Last updated
Save as PDF

Page ID: 122968

\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

After completing this lab, the student will be able to perform the following objectives:

DISCUSSION

Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony.
State and define the three types of growth that may be seen in a broth culture.
Define the following terms: obligate aerobe, microaerophile, obligate anaerobe, aerotolerant anaerobe, and facultative anaerobe.
Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.
Define the following terms: chromogenic, water-soluble pigment, and water-insoluble pigment.

PROCEDURE

1. Using an inoculating loop, demonstrate how to aseptically remove some inoculum from either a broth tube, slant tube, stab tube, or petri plate, and inoculate a sterile broth tube, slant tube, stab tube, or petri plate without introducing outside contamination.

2. Label all tubes and plates and place them on the shelf in the incubator corresponding to your lab section.

3. Return all class pure cultures to the instructor’s lab bench.

4. Dispose of all materials when the experiment is completed, being sure to remove all markings from the glassware. Place all culture tubes in the plastic baskets in the biohazard hood and all petri plates in the buckets in the biohazard hood.

RESULTS

1. Recognize and identify the following types of growth in a broth culture: pellicle, turbidity, sediment, and any combination of these.

2. State the color and water-solubility of pigment seen on a plate culture of a chromogenic bacterium.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)