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9.7: Results

  • Page ID
    123423
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    1. In the table below, describe the appearance of Candida albicans and Saccharomyces cerevisiae on Saboraud Dextrose agar.

    Also in the table below, describe the appearance of Candida albicans and Saccharomyces cerevisiae on Mycosel agar.

    Yeast SDA Mycosel agar
    Candida albicans    
    Saccharomyces cerevisiae    

    2. Remove the lid of the Rice Extract agar plate and put the plate on the stage of the microscope. Using your yellow-striped 10X objective, observe an area under the coverslip that appears "fuzzy" to the naked eye. Reduce the light by moving the iris diaphragm lever almost all the way to the right. Raise the stage all the way up using the coarse focus (large knob) and then lower the stage using the coarse focus until the yeast comes into focus. Draw the hyphae, blastoconidia, and chlamydoconidia. See lab 1 for focusing instructions using the 10X objective.

    sqlm.gif

    Candida albicans producing hyphae, blastoconidia, and chlamydoconidia on Rice Extract agar

    3. Observe and make drawings of the demonstration yeast slides.

    sqlm_qio9.gif
    Direct stain of
    Saccharomyces cerevisiae

    sqlm_qkjz.gif
    Direct stain of
    Candida albicans

    sqlm_aln9.gif
    Candida albicans from thrush
    specimen

    sqlm_3kx7.gif
    Candida albicans
    in lung tissue

    sqlm_o2gh.gif
    India ink preparation of
    Cryptococcus neoformans

    sqlm_ofdh.gif
    Pneumocystis jiroveci
    cyst in lung tissue

     

    Contributors and Attributions

    • Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)

     


    9.7: Results is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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