5.5: Performance Objectives for Lab 5

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After completing this lab, the student will be able to perform the following objectives:

A. INTRODUCTION TO STAINING

1. Describe the procedure for heat fixation and methanol fixation.

2. Define the following: acidic dye, basic dye, direct stain, and indirect stain.

3. State in chemical and physical terms the principle behind direct staining and the principle behind indirect staining.

B. DIRECT STAINING

PROCEDURE

1. Transfer a small number of bacteria from an agar surface or a broth culture to a glass slide and fix the preparation to the slide.

2. Prepare a direct stain when given all the necessary materials.

RESULTS

1. Recognize a direct stain preparation when it is observed through a microscope and state the shape and arrangement of the organism.

C. INDIRECT STAINING

PROCEDURE

1. Perform an indirect stain when given all the necessary materials.

2. State why the dye is not washed off when doing an indirect stain.

RESULTS

1. Recognize an indirect stain preparation when it is observed through a microscope and state the shape and arrangement of the organism.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)