8.7: Performance Objectives for Lab 8

Last updated
Save as PDF

Page ID: 123383

\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

After the completion of this lab, the student will be able to complete the following objectives:

INTRODUCTION

1. State the chemical nature and function of enzymes.

2. Define endoenzyme and exoenzyme.

A. STARCH HYDROLYSIS

1. Describe a method of testing for starch hydrolysis and state how to interpret the results.

1. Interpret the results of starch hydrolysis on a Starch agar plate that has been inoculated, incubated, and flooded with iodine.

B. PROTEIN HYDROLYSIS

1. Describe a method of testing for casein hydrolysis and state how to interpret the results.

1. Interpret the results of casein hydrolysis on a Skim Milk agar plate after it has been inoculated and incubated.

C. FERMENTATION OF CARBOHYDRATES

1. Name the general end products which may be formed as a result of the bacterial fermentation of sugars and describe how these end products change the appearance of a broth tube containing a sugar, the pH indicator phenol red, and a Durham tube.

1. Interpret the carbohydrate fermentation results in tubes of Phenol Red Carbohydrate broth containing a Durham tube after it has been inoculated and incubated.

D. INDOLE AND HYDROGEN SULFIDE PRODUCTION

1. State the pathway for the breakdown of tryptophan to indole. 2. State the pathway for the detection of sulfur reduction in SIM medium.

3. State three reactions that may be tested for in SIM medium and describe how to interpret the results.

1. Interpret the hydrogen sulfide and indole results in a SIM medium tube after inoculation, incubation, and addition of Kovac's reagent.

E. CATALASE ACTIVITY

1. State the function of the enzyme catalase and describe a method of testing for catalase activity.

1. Interpret the results of a catalase test after adding hydrogen peroxide to a plate culture of bacteria.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)