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2.3: Using a bacto-Incinerator to Sterilize the Inoculating Loop

  • Page ID
    122945
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    1. Sterilize the inoculating loop by placing it in the microincinerator for 10 seconds. (see Fig. \(\PageIndex{1}\)).

    Photograph showing how to sterilize the inoculating loop in a microincinerator.
    Figure \(\PageIndex{1}\): Using a Bacto-incinerator to Sterilize the Inoculating Loop. Sterilize the inoculating loop by placing it in the bacto-incinerator for 10 seconds. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    2. Lift the lid of the culture plate slightly and stab the loop into the agar away from any growth to cool the loop.

    3. Scrape off a small amount of the organisms and immediately close the lid (see Fig. \(\PageIndex{2}\)).

    Photograph showing how to scrape bacteria off of a petri plate.
    Figure \(\PageIndex{2}\): Removing Bacteria from a Petri Plate. Before removing bacteria from the petri plate, first cool the loop by sticking it into the agar away from any growth. Scrape off a small amount of bacteria. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    Contributors and Attributions

    • Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)


    2.3: Using a bacto-Incinerator to Sterilize the Inoculating Loop is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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