9.8: Performance Objectives for Lab 9

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After completing this lab, the student will be able to perform the following objectives:

DISCUSSION

1. Define mycology and mycosis.

2. State three ways fungi may be beneficial to humans and three ways they may be harmful.

1. Describe the typical appearance of a yeast cell and its usual mode of reproduction.

2. Describe yeasts in terms of their oxygen requirements.

3. State two ways the yeast Saccharomyces is beneficial to humans.

4. Name three yeasts that commonly infect humans.

5. Name four common forms of candidiasis.

6. Describe two conditions that may enable Candida to cause severe opportunistic systemic infections.

7. Describe pseudohyphae, hyphae, blastoconidia (blastospores), and chlamydoconidia (chlamydospores).

8. State the usefulness of Saboraud Dextrose agar, Mycosel agar, and Rice Extract agar.

9. State how Cryptococcus neoformans is transmitted to humans, where in the body it normally infects, and possible complications.

10. State the primary method of identifying Cryptococcus neoformans when causing cryptococcal meningoencephalitis.

11. State what disease is caused by Pneumocystis jiroveci and indicate several predisposing conditions a person is normally seen to have before they contract the disease.

12. Name an infection caused by Malassezia globosa.

RESULTS

1. Describe the appearance of Saccharomyces cerevisiae and Candida albicans on Saboraud Dextrose agar and on Mycosel agar.

2. When given a plate of Mycosel agar showing yeast-like growth and a plate of Rice Extract agar showing hyphae, blastosconidia (blastospores), and chlamydoconidia (chlamydospores), identify the organism as Candida albicans.

3. Recognize the following observed microscopically:

a. Saccharomyces cerevisiae and Candida albicans as yeasts in a direct stain preparation
b. A positive specimen for thrush by the presence of budding Candida albicans
c. Cryptococcus neoformans in an India ink preparation
d. Pneumocystis jiroveci in lung tissue

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)