17.8: Performance Objectives for Lab 17

Last updated
Save as PDF

Page ID: 122763

\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

After completing this lab, the student will be able to perform the following objectives:

A. INTRODUCTION TO THE CONTROL OF MICROORGANISM

1. Define the following terms: sterilization, disinfection, decontamination, static, and cidal.

B. TEMPERATURE

1. State whether moist or dry heat is more effective in controlling microorganisms, and indicate why.

2. State specifically how moist heat kills microorganisms.

3. State two methods of applying moist heat.

4. Briefly describe the process of autoclaving (pressure, time, and temperature).

5. State whether or not boiling is an effective means of sterilization, and indicate why.

6. State specifically how dry heat kills microorganisms.

7. State two methods of applying dry heat.

8. Define pasteurization.

9. State whether low temperature has a static or cidal effect on microorganisms, and indicate why.

C. DESICCATION

1. State whether desiccation has a static or a cidal effect on microorganisms, and indicate how it affects the cell.

D. OSMOTIC PRESSURE

1. Describe osmosis in terms of water flow through a semipermeable membrane.

2. Define the following terms: hypotonic, hypertonic, isotonic, plasmoptysis, and plasmolysis.

3. State why a hypotonic environment does not normally harm bacteria.

4. Describe how bacterial growth is inhibited in jams and salt-cured meats.

5. State whether hypertonicity has a static or a cidal effect on microorganisms.

E. RADIATION

1. State how the wavelength and the length of exposure influence the bacteriocidal effect of UV light.

2. Describe specifically how UV light kills microorganisms.

3. State why UV light is only useful as a means of controlling surface contaminants and give several practical applications.

4. Describe how ionizing radiation kills microorganisms and state several common applications.

F. FILTRATION

1. State the concept behind sterilizing solutions with micropore membrane filters.

2. State why filters are preferred over autoclaving for such materials as vaccines, antibiotic solutions, sera, and enzyme solutions.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)