12.7: Performance Objectives for Lab 12

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After completing this lab, the student will be able to perform the following objectives:

A. ENTEROBACTERIACEAE: GLUCOSE FERMENTING, GRAM-NEGATIVE, ENTERIC BACILLI

1. Name the bacterial family to which the most commonly encountered organisms isolated from clinical specimens belong.

2. List five characteristics used to place bacteria into the family Enterobacteriaceae.

3. State what infections are caused by Salmonella and by Shigella and how they are transmitted to humans.

4. Name four strains of Escherichia coli that may infect the gastrointestinal tract.

5. Name five genera of Enterobacteriaceae considered as common opportunistic pathogens, state their normal habitat, and list four common types of opportunistic infections that they all may cause.

6. Name several predisposing factors that make one more susceptible to urinary tract infections.

7. In terms of CFUs, state the laboratory culture standards for a urinary tract infection.

8. Define nosocomial infection.

9. State the significance of endotoxins in infections caused by many of the Enterobacteriaceae.

10. Discuss the significance of R-plasmids in our attempts to treat infections caused by the Enterobacteriaceae.

B. PSEUDOMONAS AND OTHER GLUCOSE NON-FERMENTING, GRAM-NEGATIVE BACILLI

1. Name the most common glucose non-fermenting Gram-negative rod that infect humans and list five types of opportunistic infections it may cause.

2, State 3 infections being caused with increased frequency by Acinetobacter. .

C. ISOLATION OF ENTEROBACTERIACEAE AND PSEUDOMONAS

1. State the usefulness of MacConkey agar and Cetrimide agar for the isolation of Enterobacteriaceae and Pseudomonas.

D. DIFFERENTIATING BETWEEN THE ENTEROBACTERIACEAE AND PSEUDOMONAS

1. State how to differentiate Pseudomonas aeruginosa from the Enterobacteriaceae using the following tests:

a. oxidase test

b. production of pigment and fluorescent products

c. odor

E. IDENTIFYING THE ENTEROBACTERIACEAE USING THE ENTEROPLURI-TEST

1. Briefly describe the EnteroPluri-Test .

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)