6.4: Performance Objectives for Lab 6

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After completing this lab, the student will be able to perform the following objectives:

DISCUSSION

1. State why the Gram stain is said to be a differential stain.

2. Describe the differences between a Gram-positive and a Gram-negative cell wall.

3. Describe a theory as to why Gram-positive bacteria retain the crystal violet-iodine complex while Gram-negatives become decolorized.

4. Describe three conditions that may result in a Gram-positive organism staining Gram-negatively.

1. State the procedure for the Gram stain.

2. Perform a Gram stain when given all the necessary materials.

1. Determine if a bacterium is Gram-positive or Gram-negative when microscopically viewing a Gram stain preparation and state the shape and arrangement of the organism.

1. State the chemical nature and major functions of bacterial capsules.

RESULTS

1. Recognize capsules as the structures observed when microscopically viewing a capsule stain preparation.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)