11.5: Viral Specificity Procedure

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Materials

Trypticase Soy agar plates (2)

Trypticase Soy broth cultures of 4 unknown bacteria labelled #1, #2, #3, and #4; suspension of Coliphage T4.

PROCEDURE (to be done in groups of three)

1. Using a wax marker, draw a line on the bottom of both Trypticase Soy agar plates dividing them in half. Number the 4 sectors 1, 2, 3, and 4, to correspond to the 4 unknown bacteria.

2. Draw a circle about the size of a dime in the center of each of the 4 sectors (see Fig. 1).

Fig \(\PageIndex{1}\): Viral Specificity	Fig. \(\PageIndex{2}\): Label your 2 TSA plates as shown below.

Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0	Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0

3. Using a sterile inoculating loop, streak unknown bacterium #1 on sector 1 of the first Trypticase Soy agar plate by streaking the loop through the circle you drew. Be careful not to streak into the other half of the plate.

4. Streak unknown bacterium #2 on sector 2 of the first Trypticase Soy agar plate.

5. Streak unknown bacterium #3 on sector 3 of the second Trypticase Soy agar plate.

6. Streak unknown bacterium #4 on sector 4 of the second Trypticase Soy agar plate.

7. Have your instructor add 1 drop of concentrated Coliphage T4 to each sector in the area outlined by the circle.

8. Incubate the 2 TSA plates right side up and stacked in the petri plate holder on the shelf of the 37°C incubator corresponding to your lab section until the next lab period.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)