15.5: Performance Objecties for Lab 15

Last updated
Save as PDF

Page ID: 122724

\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

After completing this lab, the student will be able to perform the following objectives:

DISCUSSION

1. Name three common clinically important species of Staphylococcus and state which species is most pathogenic.

2. State the sources and the portal of entry for most Staphylococcus aureus infections.

3. Name and describe three types of abscesses caused by Staphylococcus aureus.

4. Name four systemic Staphylococcus aureus infections.

5. State the significance of Staphylococcus aureus enterotoxin, the exotoxin TSST-1, and the exotoxin exfoliatin.

6. Name the infection normally caused by Staphylococcus saprophyticus.

7. Name the types of infections most commonly caused by coagulase-negative staphylococci other than Staphylococcus saprophyticus.

ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCI

1. State the Gram reaction and morphology of all staphylococci.

2. Describe the typical reactions of S. aureus, S. epidermidis, and S. saprophyticus on each of the following media:

a. Blood agar (pigment, hemolysis, novobiocin resistance)

b. Mannitol Salt agar (for mannitol fermentation)

c. coagulase test with citrated rabbit plasma

d. Staphyloslide® test for bound coagulase and/or Protein A

RESULTS

1. Recognize staphylococci in a Gram stain preparation.

2. Recognize an organism as Staphylococcus aureus and state the reasons why after seeing the results of the following:

a. a Blood agar plate with a novobiocin disc

b. a Mannitol Salt agar plate

c. a tube of citrated rabbit plasma

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)