19.3: Procedures and Results

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1. Observe the prepared slides of the following parasitic protozoans and compare them with the indicated figures in this lab exercise.

a.The Sarcodina

Fecal smear containing cysts of Entamoeba histolytica (the cause of amoebic dysentery). Note that it contains several nuclei.

b. The Mastigophora

1. Blood smear containing Trypanosoma gambiense (the cause of African sleeping sickness). Note the nucleus, the undulating membrane, and the red blood cells in the background.

2. Fecal smear containing cysts and/or trophozoites of Giardia lamblia (the cause of giardiasis). Note the bilateral symmetry and macronuclei of the organism which look like "eyes."

3. Vaginal discharge containing Trichomonas vaginalis (the cause of genitourinary trichomoniasis). Note the bundle of flagella, the undulating membrane, and the nucleus.

c. The Ciliophora

Fecal smear containing Balantidium coli (the cause of balantidiasis). Note the large dumbbell-shaped macronucleus.

d. The Sporozoa

1. Sporozoites of Plasmodium from the salivary glands of an infected mosquito.

2. Blood smear containing red blood cells infected with merozoites of Plasmodium (the cause of malaria).

2. Observe the prepared slides of the following parasitic helminths and compare them with the indicated figures in this lab exercise.

a. The Nematodes (roundworms)

1. Fecal smear containing ova of Ascaris lumbricoides (the cause of ascariasis). Note the "bumpy" edge of the ova. The life cycle is shown in Fig. 10.

2. Ascaris lumbricoides larva. Note the organ systems.

3. Fecal smear containing ova of Enterobius vermicularis (pinworm). Note the "smooth" edge of the ova. The life cycle is shown in Fig. 12.

4. Enterobius vermicularis larva. Note the organ systems.

5. Muscle tissue containing encysted larvae of Trichinella spiralis (the cause of trichinosis). Note the spiral-shaped larva within the cyst. The life cycle is shown in Fig. 14.

b. The Cestodes (tapeworms)

1. Scolex of Taenia pisiformis (dog tapeworm). Note hooks and suckers.

2. Proglottids of the tapeworm Taenia pisiformis.

3. Gravid proglottid of Taenia pisiformis. Note the uterus and ova. Also see Fig. 16A.

c. The Trematodes (flukes)

1. Fasciola hepatica (liver fluke). Note the organ systems.

2. Schistosoma mansoni (blood fluke). Note the oral and ventral suckers. See Fig. 24.

3. Observe the preserved helminths.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)