3.7: Performance Objetives for Lab 3

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After completing this lab, the student will be able to complete the following objectives:

DISCUSSION

1. Given a mixture of a Gram-positive and a Gram-negative bacterium and plates of Columbia CNA, MacConkey, and Trypticase Soy agar, describe the steps you would take to eventually obtain pure cultures of each organism.

2. Define: selective medium, differential medium, enrichment medium, and combination selective-differential medium.

3. State the usefulness of Columbia CNA agar and MacConkey agar.

4. Describe how each of the following would appear when grown on MacConkey agar:

a. Escherichia coli
b. Enterobacter aerogenes
c. Salmonella

PROCEDURE

1. Using the streak plate method of isolation, obtain isolated colonies from a mixture of microorganisms.

2. Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures.

RESULTS

1. When given a plate of Columbia CNA agar or MacConkey agar showing discrete colonies, correctly interpret the results.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)