17.6: Filtration
- Page ID
- 122893
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Microbiological membrane filters provide a useful way of sterilizing materials such as vaccines, antibiotic solutions, animal sera, enzyme solutions, vitamin solutions, and other solutions that may be damaged or denatured by high temperatures or chemical agents. The filters contain pores small enough to prevent the passage of microbes but large enough to allow the organism-free fluid to pass through. The liquid is then collected in a sterile flask (Fig. \(\PageIndex{1}\)). Filters with a pore diameter from 25 nm to 0.45 µm are usually used in this procedure. Filters can also be used to remove microorganisms from water and air for microbiological testing (see Appendix E).
