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14.11: Results for Case Study #2

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    Case Study Lab Report for Lab 14: Streptococcus and Enterococcus

    The concept behind the case studies presented in Lab 14 used to illustrate the genus Streptococcus and the genus Enterococcus is for you and your lab partners as a group to:

    1. Determine whether or not the patient in case study #1 has streptococcal pharyngitis.

    2. Come up with a valid diagnosis of the infectious disease in case study #2 and identify the bacterium causing that infection.

    3. Support your group’s diagnose based on:

    a. Any relevant facts in the patient’s history.(A reliable on-line source will be used to support this.)
    b. The patient’s signs and symptoms. (A reliable on-line source will be used to support this.)
    c. Each of the individual lab tests given in your case study.
    d. All microbiological lab tests you performed as part of the project.

    The due date for this report can be found on the class calendar. Your grade for this lab is based on the completeness of your report and written evidence of the critical thinking process that went into making and supporting your diagnosis. Remember, you are trying to convince your instructor that you understand how the diagnosis was made by supporting that diagnosis with data.

    Grading:

    The lab 14 Lab Report is worth 21 points each.

    These case studies are based in part on your in-class participation as part of your group. Therefore:

    a. If you were not in lab when the inoculations with your unknown were performed, 3 points will be deducted from your Lab Report score for labs 12, 14, and 15; 6 points from your Lab Report score for the Final Project.
    b. If you were not in lab when the results of your lab tests were observed, 3 points will be deducted from your Lab Report score for labs 12, 14, and 15; 6 points from your Lab Report score for the Final Project..
    c. For each day your Lab Report is late, 2 points will be deducted from your Lab Report score for labs 12, 14, and 15; 4 points from your Lab Report score for the Final Project.

    Be sure to handle all the bacterial cultures you are using in lab today as if they are pathogens! Be sure to wash and sanitize your hands well at the completion of today’s lab.

    Also, make sure you observe the results of of someone in your lab who had an unknown different from yours in case study #1. The Performance Objectives for Lab 14 tell you what you are expected to be able to do on the practical.

    Each member of the group must:

    1. Print a copy of each of the two rubrics from the links above.

    2. Print and fill out a copy of the Team Member Evaluation Form from the link above.

    3. Staple them together and hand them in to me the day your Lab 14 Case Study Lab Report is due.

    For more information on writing your Lab Report, see Course evaluation for Lab (Core labs, case studies, lab quizzes) under Course Info in the menu of the BIOL 230 website.

    Lab Report

    Your Name:

    Others in your group:

    Lab section:

    Date:

    A 57-year old diabetic male hospitalized following hip replacement surgery has had an indwelling urinary catheter inserted for 8 days. He presents with suprapubic discomfort. His blood pressure is normal and he does not have fever, chills, or flank pain. There is no costovertebral angle (CVA) tenderness. A complete blood count (CBC) shows leukocytosis with a left shift. A urine dipstick shows a positive leukocyte esterase test, a negative nitrite test, 30mg of protein per deciliter, and red blood cells in the urine. Microscopic examination of centrifuged urine shows 50 white blood cells, as well as 20 bacteria and 5 red blood cells per high-power field.

    1. Patient’s history and predisposing factors

    Read the case study. Explain how any relevant parts of the patient’s history contributed to your diagnosis of the type of infectious disease seen here. The patient's history refers to anything given in the case study prior to that patient seeking medical attention for the current medical condition. You are urged to use the computers in lab to search reliable medically oriented Internet sources to support this. Reliable sources you might consider are Medscape (http://emedicine.medscape.com/infectious_diseases) and The Centers for Disease Control and Prevention (CDC) at http://www.cdc.gov/. Cite any sources you use at the end of this Patient's History section in APA style (https://www.scribbr.com/apa-examples/website/). Also see appendix F (SIRS and Sepsis) in your lab manual for an indication of whether or not the patient has SIRS.

    The patient's history should suggest a general type of infectious disease that is present, such as a urinary tract infection, a wound infection, gastroenteritis, pharyngitis, pneumonia, septicemia, etc. Do not look up the bacterium you eventually identify as the cause of this infectious disease. You don't know the causative bacterium at this point. You need to determine the general type of infection in order to determine what microbiological tests to perform to identify the bacterium causing the infection. Search at least one medically-oriented reference article from a reliable site such as Medscape and use this article to support your diagnosis of the type of infectious disease seen here. Don't forget to cite any sources you used in APA style directly under this Patient's History and Patient's Symptoms sections of this Lab Report.

    2. Patient’s signs and symptoms

    Read the case study. Explain how the patient’s signs and symptoms contributed to your diagnosis of the type of infectious disease seen here. You are urged to use the computers in lab to search reliable medically oriented Internet sources to support this. Reliable sources you might consider are Medscape (http://emedicine.medscape.com/infectious_diseases) and The Centers for Disease Control and Prevention (CDC) at http://www.cdc.gov/. Cite any sources you use at the end of this Patient's Symptoms section in APA style (http://www.apastyle.org/).

    The patient's signs and symptoms should suggest a general type of infectious disease that is present, such as a urinary tract infection, a wound infection, gastroenteritis, pharyngitis, pneumonia, septicemia, etc. Do not look up the bacterium you eventually identify as the cause of this infectious disease. You don't know the causative bacterium at this point. You need to determine the general type of infection in order to determine what microbiological tests to perform to identify the bacterium causing the infection. Search at least one medically-oriented reference article from a reliable site such as Medscape and use this article to support your diagnosis of the type of infectious disease seen here. Don't forget to cite any sources you used in APA style directly under this Patient's History and Patient's Symptoms sections of this Lab Report.

    3. Vocabulary list for medical terms used in the case study under signs and symptoms

    List and define any medical terms used in your case study that describe the patients’s signs and symptoms that the average person not in the medical profession might not know.

    4. Results of laboratory test given in the case study

    List each lab test given in the case study that are done in a lab, such as total white blood count, differential white blood cell count, urinalysis, and X-ray, and explain how the results of that test helps to contribute to your diagnosis. The CBC and urinalysis tests are described in Appendix C and Appendix D of this lab manual.

    5. Microbiological lab tests you performed in Lab 14

    a. Gram stain and catalase test results

    Give the Gram reaction (Gram-positive or Gram negative and how you reached this conclusion) and the shape and arrangement of the unknown you were given. Because Enterococci and Staphylococci can sometimes look similar in Gram stains done from a plate culture, perform a catalase test on your unknown to help differentiate an Enterococcus from a Staphylococcus. State how this contributed to your decision as to which microbiological tests and/or media to use next. The Gram stain is discussed in Lab 6; the catalase test in Lab 8.

    c. Bile Esculin Azide agar

    Note

    Videos lesson:

    Give the results of the Bile Esculin Azide agar plate you inoculated with the unknown you were given, and how you reached those conclusions. State how this contributed to your final diagnosis of the bacterium causing this infection. The possible results for Bile Esculin Azide agar were discussed earlier in this lab.

     

    Genus of bacterium: __________________________________

    Infection: _____________________________

    Contributors and Attributions

    • Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)

     

     

    14.11: Results for Case Study #2 is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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