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2.4: Transferring the Inoculum into a Broth Tube

  • Page ID
    122946
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    1. Pick up the sterile broth tube and remove the cap with the little finger of your loop hand (see Fig. \(\PageIndex{1}\)). Do not set the cap down.

    Photograph showing how to remove the cap of a sterile broth tube.
    Figure \(\PageIndex{1}\):Removing the Cap of the Sterile Broth Tube. Using the little finger of your loop hand, remove the cap of the sterile broth tube. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    2. Place the lip of the culture tube at the opening of the microincinerator for 2-3 seconds (see Fig. \(\PageIndex{2}\)).

    Photograph showing heating the lip of the tube.
    Figure \(\PageIndex{2}\): Heating the Lip of the Tube. Place the lip of the tube at the opening of the bacto-incinerator for 2-3 seconds. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    3. Place the loopful of inoculum into the broth, and withdraw the loop (see Fig. \(\PageIndex{3}\)). Do not lay the loop down!

    Photograph showing the inoculation of a sterile broth tube with the bacteria from a culture tube
    Figure \(\PageIndex{3}\): Inoculating a Sterile Broth Tube with the Bacteria from the Culture Tube. Place bacteria from your pure culture into the sterile broth tube. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    4. Again place the lip of the culture tube at the opening of the microincinerator for 2-3 seconds (see Fig. \(\PageIndex{4}\)).

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    Figure \(\PageIndex{4}\): Heating the Lip of the Tube. Place the lip of the tube at the opening of the bacto-incinerator for 2-3 seconds. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    5. Replace the cap (see Fig. \(\PageIndex{5}\)).

    Photograph showing replacing the cap of the tube.
    Figure \(\PageIndex{5}\): Replacing the cap of the Tube. Replace the cap of the pure culture tube. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    6. Resterilize the inoculating loop by placing it in the microincinerator for 10 seconds (see Fig. \(\PageIndex{6}\)). Now you may lay the loop down until it is needed again.

    Photograph showing using a microincinerator to sterilize the inoculating loop.
    Figure \(\PageIndex{6}\): Using a Bacto-incinerator to Sterilize the Inoculating Loop. Sterilize the inoculating loop by placing it in the bacto-incinerator for 10 seconds. (Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0)

    Contributors and Attributions

    • Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)


    2.4: Transferring the Inoculum into a Broth Tube is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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