10.5: Procedure

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PROCEDURE (to be done individually)

A. COMMON MOLDS

1. Using your microscope, observe a prepared slide of Penicillium. Focus first using the yellow-striped 10X objective (100X magnification) and then rotate to the 40X objective (400X magnification). Note the type of asexual spores produced and on what they are borne. Focusing instructions when using the 10X objective can be found in Lab 1.

2. Using your microscope and using the yellow-striped 10X objective (100X magnification), observe a prepared slide of Aspergillus. Note the type of asexual spores produced and on what they are borne.

3. Using your microscope and using the yellow-striped 10X objective (100X magnification), observe a prepared slide of Rhizopus. Note the type of asexual spores produced and on what they are borne.

4. Observe the prepared slide showing the zygospore of Rhizopus produced during sexual reproduction.

B. DERMATOPHYTES

1. Observe the dermatophyte Microsporum growing on DTM. Note the red color from production of alkaline end products indicating that it is breaking down the keratin in the agar. This indicates that the organism is a dermatophyte.

2. Microscopically observe macroconidia and microconidia of Microsporum.

C. DIMORPHIC FUNGI

1. Observe the prepared slide of Coccidioides immitis arthroconidia.

2. Observe the pictures of Coccidioides immitis showing the mold form with arthroconidia seen in the soil as well as the endosporulating spherule form seen in the lungs.

3. Observe the pictures of Histoplasma capsulatum showing the mold form with tuberculate macroconidia and microconidia seen in the soil as well as yeast form seen in the lungs.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)