Irvine Valley College
- Page ID
- 155982
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)- Biotechnology: Basic Lab Techniques (BIOT 173 LAB MANUAL)
- Front Matter
- 1: Introduction to Biotechnology
- 2: Scientific Method
- 3: Lab Safety
- 4: Lab Notebook
- 5: Measurements and Unit Conversion
- 6: Significant Figures and Standard Mass
- 7: Water - The Solvent of Life
- 8: Micropipetting
- 9: Dilutions and Spectrophotometry
- 10: Enzymes and pH Buffer
- 11: Creating Buffer Solutions
- 12: Advance Solution Preparation
- 13: Final Practicum
- 14: Materials List
- Back Matter
- Biotechnology Techniques: From Theory to Practice (BIOT173 at IVC)
- Front Matter
- 1: Biotechnology- From DNA Manipulation to Innovative Solutions
- 2: Regenerative Medicine and Genomics- A Comprehensive Exploration
- 3: Navigating the Lifecycles of Biotechnological Products
- 4: A Comprehensive Exploration of Safety Protocols and Regulatory Frameworks
- 5: Lab Technician's Guide to Accuracy, Precision, and Reliability
- 6: Principles and Applications of Weight Measurement
- 7: Precision in Liquid Handling of Serological Pipettes
- 8: Biosafety Protocols and Hazard Mitigation in Laboratory
- Back Matter
- IVC Microbiology Lab Manual
- This is a Microbiology lab manual that goes with a separate textbook for IVC students for a 5 unit class. This lab manual provides students with fundamental skills and concepts of the structure and physiology of non-disease and disease producing microorganisms with particular attention to bacteria. Basic techniques for culturing, staining, counting and identifying microorganisms. Designed to meet the requirement to enter one of the medical fields as well as general education.
- Front Matter
- 1: INTRODUCTION. SAFETY AND LAB EXERCISE 1
- 2: EXPOSURE PLATES
- 3: DECONTAMINATION
- 4: MICROSCOPY
- 5: MEASURING WITH A MICROSCOPE
- 6: STERILE TECHNIQUE
- 7: SMEAR AND SIMPLE STAIN
- 8: GRAM STAIN
- 9: ISOLATION BY STREAK PLATE
- 10: BACTERIAL GROWTH CURVE
- 11: EPIDEMIC
- 12: OUTBREAK
- 13: SURVEY OF FUNGI
- 14: ALGAE
- 15: PROTOZOA
- 16: TEMPERATURE EFFECTS
- 17: ANTIBIOTICS
- 18: ANTISEPTICS AND DISINFECTANTS
- 19: ALCOHOL FERMENTATION
- 20: SKIN AND UPPER RESPIRATORY BACTERIA
- 21: ANTIBIOTIC PRODUCING MICROBES IN SOIL
- 22: VIRAL PLAQUE ASSAY
- 23: ACID FAST STAIN
- 24: CAPSULE STAIN
- 25: BACTERIAL COUNTS ON FOOD
- 26: ANAEROBIC GROWTH
- 27: ENDOSPORE STAIN
- 28: EFFECTS OF UV LIGHT ON BACTERIA
- 29: BLOOD TYPING
- 30: TESTING YOUR KITCHEN
- 31: METABOLIC TESTING
- Back Matter
- Lab manual: Molecular biology and Genetic Engineering (Biot 275)
- Front Matter
- Activity 1-1 – Data on Human Traits
- Activity 1-2 - Human Genetic Inheritance and Diseases
- Activity 1-3 - Genetic evolution and Identifying Homologs
- Activity 1-4 - PowerPoint Presentation on the Bioinformatic Evolution and function of proteins
- Activity 2-0 - Introduction to Plasmid and Enzyme p450
- Activity 2-1 - Plasmids Isolation
- Activity 2-2 - DNA Analysis via Restriction Enzymes
- Project 2-3 - Identifying Plasmid Restriction Mapping Using Agarose Gel Electrophoresis
- Activity 2-4 - Bacterial Transformation with Engineered Plasmid
- Activity 3-1 - Identifying Genetically Modified Organisms Through DNA Analysis
- Activity 3-2 - Primer Design and Barcoding via Bioinformatics
- Activity 4-1 - RNA Extraction via Guanidine Thiocyanate and Phenol
- Activity 4-2 - Reverse Transcription Polymerase Chain Reaction
- Activity 4-3 - Real-Time Quantitative Polymerase Chain Reaction
- Activity 4-4 - DNA Sequencing with SeqStudio Genetic Analyzer
- Back Matter
- Lab manual : Protein Biomanufacturing and Biochemistry (Biot274 @IVC)
- Front Matter
- 1: Activity 1-1 - Protein Extraction Using Bacterial Cell Lysis
- 2: Activity 1-2 - Bradford Assay – Measuring Protein Concentration
- 3: Activity 1-3 - Beer’s Law Spectrophotometric Analysis
- 4: Activity 1-4 - Analysis of Enzymatic Activities of Cell-Free Extracts
- 5: Activity 2-1 - Purification via Ammonium Sulfate Saturation of Cell-Free Extract
- 6: Activity 2-2 - Dialysis for Enzyme Purification
- 7: Activity 2-3 - Size Exclusion Gel Filtration Purification of Enzymes
- 8: Activity 2-4 - Verification of Protein Purification via Polyacrylamide Gel Electrophoresis
- 9: Activity 3-0 - Project Instructions - Investigating Enzyme Kinetics
- 10: Activity 3-1 - Michaelis-Mentent Analysis of Substrate Concentration on Reaction Rate
- 11: Activity 3-2 - Determining the IC₅₀ of Inhibitor
- 12: Activity 3-3 - Identifying the Type of Enzyme Inhibition
- Back Matter
- Lecture: Protein, amino acids, enzymes, and kinetics (Biot274 @ IVC)
- Front Matter
- 1: Chemistry
- 2: Macromolecules
- 3: Water and Buffer
- 4: Photosynthesis and Plant Adaptation
- 5: Anaerobic and Aerobic Respiration
- 6: Amino Acids and Protein structures
- 7: Enzymes and Kinetics
- 8: Inhibitors
- 9: Carbohydrates
- 10: Metabolism and Glycolysis
- 11: Glycogenesis and Gluconeogenesis
- 12: TCA cycle
- 13: Electron Transport Chain
- 14: Lipid
- 15: Lipids Catabolism
- 16: Lipids Anabolism
- Back Matter

