Lab manual : Protein Biomanufacturing and Biochemistry (Biot274 @IVC)
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Front Matter
1: Activity 1-1 - Protein Extraction Using Bacterial Cell Lysis
2: Activity 1-2 - Bradford Assay – Measuring Protein Concentration
3: Activity 1-3 - Beer’s Law Spectrophotometric Analysis
4: Activity 1-4 - Analysis of Enzymatic Activities of Cell-Free Extracts
5: Activity 2-1 - Purification via Ammonium Sulfate Saturation of Cell-Free Extract
6: Activity 2-2 - Dialysis for Enzyme Purification
7: Activity 2-3 - Size Exclusion Gel Filtration Purification of Enzymes
8: Activity 2-4 - Verification of Protein Purification via Polyacrylamide Gel Electrophoresis
9: Activity 3-0 - Project Instructions - Investigating Enzyme Kinetics
10: Activity 3-1 - Michaelis-Mentent Analysis of Substrate Concentration on Reaction Rate
11: Activity 3-2 - Determining the IC₅₀ of Inhibitor
12: Activity 3-3 - Identifying the Type of Enzyme Inhibition
Back Matter