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Biology LibreTexts

16.3: Pre-lab Questions

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    1. Define the terms transformation, recombinant plasmid, and competent cell.
    2. Investigate green fluorescent protein, online, and describe what it means to “fluoresce”. Did you find any unusual applications of genetically modifying organisms with this protein?
    3. Explain the use of antibiotic resistance as a “selectable marker”.
    4. Predict what will happen if transformed cells are fed only:
      1. Glucose
      2. Glucose and arabinose
      3. Glucose and sucrose

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