Bio 221Lab: Introduction to Microbiology (Burke)
- Page ID
- 40144
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)This lab manual provides an introduction to the biology and ubiquity of microorganisms (for example, bacteria, viruses, protozoa, fungi), including morphology, anatomy, physiology, classification, and identification. The significance and role of microorganisms in human affairs will also be explored.
Front Matter
Unit 1: Safety
Unit 2: The Metric System, Measurement, and Lab Equipment Review
Unit 3: Microscopy
Unit 4: Environmental Sampling
Unit 5: Survey of Eukaryotic Microorganisms- The Protists Algae
Unit 6: Parasitic Helminths
Unit 7: Fungi
Unit 8: Pure Cultures- Aseptic Transfer Techniques and Streak Plates for Isolation
Unit 9: Bacterial Growth Patterns- Building your Stock Cultures and Observing Culture Characteristics
Unit 10: Bacterial Growth Patterns- Direct Count, The Standard Plate Count, and Indirect Turbidimetric Methods
Unit 11: Environmental Effects on Growth- Temperature
Unit 12: Environmental Effects on Growth- pH
Unit 13: Environmental Effects on Growth- Osmotic Pressure
Unit 14: Oxygen Requirements- FTM and the Anaerobe Jar
Unit 15: Environmental Effects on Growth- Antimicrobial Sensitivity Testing
Unit 16: Transformation\(^1\)
Unit 17: Smear Prep and Simple Stains
Unit 18: Negative Stain
Unit 19: Gram Stain
Unit 20: Endospore Stain
Unit 21: Acid-Fast Stain- Kinyoun Method
Unit 22: Physiological Tests for Characterization and Identification of Bacteria
Unit 23: Unknown 1 - What is yellow, wrinkled, round,...?
Unit 24: Unknown 2- Mixed Culture
Unit 25: Bacterial Examination of Food- Standard Plate Counts
Unit 26: Bacterial Examination of Water- Multiple Tube Test, Standard Plate Count, and Membrane Filter Technique
Unit 27: Immunology- ELISA-Simulation, StaphTEX-Agglutination Reaction
Unit 28: Microbescopes and Observation of Natural Samples
Back Matter
Thumbnail: A neutrophil (a type of white blood cell, shown in blue-gray) interacting with Klebsiella pneumoniae bacteria (shown in pink). CC-BY 2.0, NIAID)