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- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/04%3A_Environmental_Sampling/4.06%3A_Conclusion_and_ResourcesConclusion What did you learn about the importance of aseptic technique and protecting your cultures? How would you apply this to a situation at home or in a hospital? Contributors and Attributions Ke...Conclusion What did you learn about the importance of aseptic technique and protecting your cultures? How would you apply this to a situation at home or in a hospital? Contributors and Attributions Kelly C. Burke (College of the Canyons)
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/28%3A_Microbescopes_and_Observation_of_Natural_Samples
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/09%3A_Bacterial_Growth_Patterns-_Building_your_Stock_Cultures_and_Observing_Culture_Characteristics/9.05%3A_Post-lab_QuestionsAnswer the following once you have completed your culture collection. Were any of your cultures contaminated? If so, give possible explanations why the culture was contaminated. Compare and contrast t...Answer the following once you have completed your culture collection. Were any of your cultures contaminated? If so, give possible explanations why the culture was contaminated. Compare and contrast the growth characteristics of the following groups of bacteria: Staphylococcus saprophyticus vs. Staphylococcus epidermidis Alcaligenes viscolactis vs. Escherichia coli vs. Citrobacter freundii Contributors and Attributions Kelly C. Burke (College of the Canyons)
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/06%3A_Parasitic_Helminths
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/15%3A_Environmental_Effects_on_Growth-_Antimicrobial_Sensitivity_Testing/15.05%3A_Post-lab_QuestionsWhat is the purpose of the Kirby-Bauer test? Where you able to measure and interpret each bacterial-antibiotic pair? If not, what were some of the issues and why do you think they occurred? How might ...What is the purpose of the Kirby-Bauer test? Where you able to measure and interpret each bacterial-antibiotic pair? If not, what were some of the issues and why do you think they occurred? How might the amount of bacteria—too much, or too little--affect the results of the test? Compare the results of the Gram negative bacteria tested, with the Gram-positive bacteria tested. You may need to look up which are G+ and G-. What are the implications of this? Burke (College of the Canyons)
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/19%3A_Gram_Stain/19.04%3A_Materials_and_ProceduresThis can cause a fair amount of grief and frustration when evaluating your stain, and may cause you to miss-identify the gram reaction. Slowly DRIP the decolorizer down the slide until it runs clear o...This can cause a fair amount of grief and frustration when evaluating your stain, and may cause you to miss-identify the gram reaction. Slowly DRIP the decolorizer down the slide until it runs clear of the slide. Do not flush the slide with a lot of the ethanol/acetone as this may over decolorize your specimen and cause you to misinterpret the results. Gently, but completely, rinse the slide with DI water. Fill in the table with the organisms you stained, including the mixed slide.
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/24%3A_Unknown_2-__Mixed_Culture/24.04%3A_Materials_and_ProceduresReceive mixed culture, label it, and record #. DO NOT discard this original culture. Subsequent Days (activities will be individual in nature, and not everyone will do the same thing on Day 2, Day 3, ...Receive mixed culture, label it, and record #. DO NOT discard this original culture. Subsequent Days (activities will be individual in nature, and not everyone will do the same thing on Day 2, Day 3, etc.): Subculture ISOLATED colonies only onto a TSA plate, TSA slant, and into a TSB tube, incubate. After incubation observe and record culture characteristics of your plate, slant, and broth. Request media and run appropriate tests on ISOLATED colonies as needed.
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/26%3A_Bacterial_Examination_of_Water-_Multiple_Tube_Test_Standard_Plate_Count_and_Membrane_Filter_Technique
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/24%3A_Unknown_2-__Mixed_Culture/24.03%3A_Pre-lab_QuestionsWhat are methods you have used in lab to separate or select for certain organisms? Describe your experiences with contaminated cultures in lab. Why will it be important to maintain a high level of ase...What are methods you have used in lab to separate or select for certain organisms? Describe your experiences with contaminated cultures in lab. Why will it be important to maintain a high level of aseptic technique during Unknown #2? List the important first steps and procedures you will take with your mixed culture. Try to list them in the order you will do them. Contributors and Attributions Kelly C. Burke (College of the Canyons)
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/24%3A_Unknown_2-__Mixed_Culture/24.02%3A_IntroductionIn this round of unknowns, you will be given a mixed culture of bacteria that will contain 2-3 bacteria. Using pure culture methods, you will separate and subculture your isolates and then work to ide...In this round of unknowns, you will be given a mixed culture of bacteria that will contain 2-3 bacteria. Using pure culture methods, you will separate and subculture your isolates and then work to identify them. You will utilize all the observable characteristics of the isolates as was done in Unknown #1-stain characteristics, cell morphology, arrangement, size, culture characteristics, and physiological test results.
- https://bio.libretexts.org/Courses/College_of_the_Canyons/Bio_221Lab%3A_Introduction_to_Microbiology_(Burke)/25%3A_Bacterial_Examination_of_Food-_Standard_Plate_Counts/25.02%3A_IntroductionMost foods are solid at least in some way, and so food must be re-suspended in water in order to perform a plate count. In addition, because most foods will not contain extremely high numbers of bacte...Most foods are solid at least in some way, and so food must be re-suspended in water in order to perform a plate count. In addition, because most foods will not contain extremely high numbers of bacteria the dilution scheme is set-up accordingly. Your group will bring in a sample of a fresh food that should be safe to eat, yet might contain bacteria. A Standard Plate Count (SPC) will be performed to determine the number of viable bacteria in the original sample.
