4. Biotechnology 2
- Page ID
- 18146
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- 4.1: Protein Purification
- A successful protein purification procedure can be nothing short of amazing. Whether you are starting off with a recombinant protein which is produced in E. coli, or trying to isolate a protein from some mammalian tissue, you are typically starting with gram quantities of a complex mixture of protein, nucleic acids, polysaccharide, etc. from which you may have to extract milligram (or microgram!) quantities of desired protein at high purity, and hopefully with high yield.
- 4.2: M13 Phage
- The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and "combinatorial" DNA and peptide libraries.
- 4.4: SELEX (Selective Evolution of Ligands by Exponential Enrichment)
- Systematic evolution of ligands by exponential enrichment (SELEX) is a combinatorial chemistry technique in molecular biology for producing oligonucleotides of either single-stranded DNA or RNA that specifically bind to a target ligand or ligands.
- 4.6: Molecular Imprinting
- A major emphasis in biotechnology is the development of synthetic recognition molecules and systems which are specific for a ligand of interest. One branch of molecular design involves the synthesis of molecules with specific stereochemical structure designed to recognize and interact with a particular ligand. In contrast, molecular imprinting is a method of constructing a molecule, with specific recognition properties, by having the ligand itself direct the assembly of the desired structure