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- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/5._Lab_Notes_Part_1/5.2%3A_SpectrophotometryEven though the sample is "greener" with higher concentrations of chlorophyll, this is not due to an increase in the intensity of the green wavelength of light; it is due to a reduction in the violet/...Even though the sample is "greener" with higher concentrations of chlorophyll, this is not due to an increase in the intensity of the green wavelength of light; it is due to a reduction in the violet/blue and orange/red wavelengths of light. The different wavelengths of light differ in the energy they carry; the energy is directly proportional to the frequency of the light (the higher the frequency, the higher the energy):
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/4._Biotechnology_2/4.5%3A_Protein-Protein_Recognition_Probed_Using_a_Yeast_Transcriptional_Activator_SystemThe Gal4 protein is not one of the enzymes involved in galactose metabolism, rather, it a appears to be a transcriptional activator protein which specifically activates the transcription of genes invo...The Gal4 protein is not one of the enzymes involved in galactose metabolism, rather, it a appears to be a transcriptional activator protein which specifically activates the transcription of genes involved in the galactose metabolic pathway. These plasmids were inserted into a host yeast (GGY1::171)which had the GAL4 gene deleted, the LACZ gene deleted, the HIS3 and LEU2 genes mutated to be non-productive and which also contained a GAL1-LACZ gene fusion (i.e.
- https://bio.libretexts.org/Courses/Wheaton_College_Massachusetts/Principles_of_Biochemistry/05%3A_Techniques_for_studying_proteins/5.03%3A_Quantification_of_Protein_ConcentrationChromo means color and genesis mean creation, so chromogenic means the "creation of color". Color means color (duh…) and metric means to measure, so colorimetric is to "measure color". Both terms refe...Chromo means color and genesis mean creation, so chromogenic means the "creation of color". Color means color (duh…) and metric means to measure, so colorimetric is to "measure color". Both terms refer to the same sort of thing in the present case - we can modify the protein sample with appropriate reagents so as to produce a color reaction (in visible spectrum) and measure protein concentration using a VIS spectrophotometer.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/4._Biotechnology_2/4.3%3A_M13_Phage_Display_LibrariesIf we can increase the expression of the wild type gene III protein, to the point where it is present in four-fold excess over the mutant, then the progeny phage will, on average, contain 1 copy of th...If we can increase the expression of the wild type gene III protein, to the point where it is present in four-fold excess over the mutant, then the progeny phage will, on average, contain 1 copy of the mutant gene III protein (and ligand binding will, by definition, be mono-valent)
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/2%3A_Bacteria/2.5%3A_Gene_and_OperonThe entire nucleic acid sequence that is necessary for the synthesis of a functional polypeptide or RNA molecule. Thus, a gene contains additional sequence information beyond that which codes for the ...The entire nucleic acid sequence that is necessary for the synthesis of a functional polypeptide or RNA molecule. Thus, a gene contains additional sequence information beyond that which codes for the amino acids in a protein or the nucleotides in an RNA molecule. The gene also contains the DNA necessary to get a particular transcript made.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/6._Lab_Notes_Part_2
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/5._Lab_Notes_Part_1/5.5%3A_Gel_Electrophoresis_of_ProteinsDue to the charged nature of the SDS molecule the proteins thus will have an approximate constant charge to mass ratiodue to the charge provided by the SDS, and will migrate through the gel at a rate ...Due to the charged nature of the SDS molecule the proteins thus will have an approximate constant charge to mass ratiodue to the charge provided by the SDS, and will migrate through the gel at a rate proportional to their molecular mass Protein gels are usually performed under denaturing conditions, meaning that the sample preparation involves heating the protein in the presence of SDS to fully unfold the protein and permit binding of SDS throughout the length of the polypeptide.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/5._Lab_Notes_Part_1/5.1%3A_Basic_practices_and_techniques_Laboratory_notebooks_Presentation_of_data_Sections_of_a_scientific_reportIn industrial (and some academic) settings, it is a requirement that another person in the lab sign off on your notebook that they have read, and can understand, your notebook entry for a particular e...In industrial (and some academic) settings, it is a requirement that another person in the lab sign off on your notebook that they have read, and can understand, your notebook entry for a particular experiment (this is associated with the legal function of the notebook). One or more paragraphs that explain the purpose of the experiment, and provide any relevant background information so that the reader is prepared to follow the narrative of the report.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/2%3A_Bacteria/2.8%3A_mRNA_TranslationThe first event of the initiation stage is the attachment of a free molecule of methionine (Met) to the end of a tRNA Met by a specific aminoacyl-tRNA synthetase. In the first part of the elongation s...The first event of the initiation stage is the attachment of a free molecule of methionine (Met) to the end of a tRNA Met by a specific aminoacyl-tRNA synthetase. In the first part of the elongation step of translation, the ribosome moves along the mRNA to position the fMet residue to the P site (peptidyl site) in the 50S subunit. Next peptide bond formation is synthesized and the tRNA in the A site (which is covalently attached to the nascent polypeptide) is translocated to the P site.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/3._Biotechnology_1
- https://bio.libretexts.org/Bookshelves/Biochemistry/Supplemental_Modules_(Biochemistry)/5._Lab_Notes_Part_1/5.3%3A_Quantification_of_Protein_ConcentrationChromo means color and genesis mean creation, so chromogenic means the "creation of color". Color means color (duh…) and metric means to measure, so colorimetric is to "measure color". Both terms refe...Chromo means color and genesis mean creation, so chromogenic means the "creation of color". Color means color (duh…) and metric means to measure, so colorimetric is to "measure color". Both terms refer to the same sort of thing in the present case - we can modify the protein sample with appropriate reagents so as to produce a color reaction (in visible spectrum) and measure protein concentration using a VIS spectrophotometer.
