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9: Investigating DNA

  • Page ID
    14975
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    • 9.1: DNA Isolation, Sequencing, and Synthesis
      The document provides a comprehensive overview of DNA technologies, focusing on DNA extraction, sequencing, and synthesis. It distinguishes between genomic and complementary DNA, describing their structures and roles. DNA sequencing methods, including Sanger sequencing and Next-Generation Sequencing (NGS), are explored, highlighting advancements and their applications in genomics.
    • 9.2: Bioinformatics
      The introduction discusses the significant impact of technological advances on science, particularly in generating and managing "omic" data. The complexities in understanding this data in biology and bioinformatics present challenges that are addressed through new computational tools and interdisciplinary approaches.
    • 9.3: Cloning and Recombinant Expression
      The document provides an overview of molecular and reproductive cloning along with genetic engineering techniques. It describes how cloning vectors, such as plasmids, cosmids, and BACs, are used to insert and express DNA in host cells. The text also covers the use of restriction enzymes, selectable markers, and reporter genes in cloning processes.
    • 9.4: DNA Microarrays
      The text provides an in-depth explanation of DNA microarrays, a technology used to measure gene expression levels or genotype different regions of a genome. DNA microarrays consist of microscopic spots of DNA sequences on a solid surface, functioning as probes that hybridize with target samples under study. The hybridization is detected via labeled targets and helps determine the abundance of nucleic acid sequences. Two types of arrays are highlighted: solid-phase and bead arrays.
    • 9.5: In Situ Hybridization
      In situ hybridization (ISH) is a technique used to locate specific DNA or RNA sequences in tissues or cells, using labeled probes. It differs from immunohistochemistry, which targets proteins. ISH is crucial for understanding gene organization and function, and includes techniques like fluorescent in situ hybridization (FISH) for chromosomal sequences. It uses labeled complementary probes and allows for precise localization and quantification of nucleic acids.
    • 9.6: References


    This page titled 9: Investigating DNA is shared under a not declared license and was authored, remixed, and/or curated by Henry Jakubowski and Patricia Flatt.