12.5.2: Procedures for Case Study #2

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After receiving a baby chicken for Easter, a 7-year-old boy is taken to the emergency room with symptoms of vomiting, nausea, non-bloody diarrhea, abdominal cramps, and a temperature of 100°F. A complete blood count (CBC) shows the WBC count to be within the reference range.

This XLD agar plate and this EnteroPluri-Test are from a stool culture from this patient.

Caution

CAUTION: TREAT THE UNKNOWN AS A PATHOGEN!. Inform your instructor of any spills or accidents. WASH AND SANITIZE YOUR HANDS WELL before leaving the lab.

MATERIALS

Demonstration XLD agar plate and EnteroPluri-Test

PROCEDURE (to be done in groups of 3)

1. Observe the following demonstrations shown below and identify the causative bacterium:

a. Fig. \(\PageIndex{1}\): An XLD agar plate. XLD agar is a selective medium used for isolating and differentiating Gram-negative enteric bacteria, especially intestinal pathogens such as Salmonella and Shigella.

b. Fig. \(\PageIndex{2}\): An EnteroPluri-Test.

Fig \(\PageIndex{1}\): Your Unknown Growing on XLD Agar for Case Study #2	Fig. \(\PageIndex{2}\): Your Unknown Growing in an EnteroPluri Test for Case Study #2

Photo courtesy of Nathan Reading, Halesowen, UK	Copyright; Gary E. Kaiser, Ph.D. The Community College of Baltimore County, Catonsville Campus CC-BY-3.0

2. Record your results in the Results section of Lab 13.

Contributors and Attributions

Dr. Gary Kaiser (COMMUNITY COLLEGE OF BALTIMORE COUNTY, CATONSVILLE CAMPUS)