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Lab 13: ELISA

  • Page ID
    24062
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    ELISA:clipboard_e1167912dbbcef12c031fbea79321ee34.png

    1. Record results of Widal test using + or - signs:

    • extremely agglutinated (+++)
    • very agglutinated (++)
    • a little agglutinated (+)
    • no agglutination ( – )

    2. Positive results should lead to agglutination (clumping) of antibody to antigen. Your titer is the lowest dilution that had a positive result. CIRCLE YOUR TITER BELOW.

    Dilution 1:20 1:40 1:80 1:160 1:320 1:640

    ELISA: enzyme-linked immunosorbent assay

    ELISA is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones using antibodies and color changes. ELISA is a common medical and research lab technique.

    clipboard_e855eb3cf7df0499209d8079397321449.png


    Lab 13: ELISA is shared under a CC BY-SA license and was authored, remixed, and/or curated by Nazzy Pakpour & Sharon Horgan.

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