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Lab 13: ELISA

Last updated

Mar 19, 2021
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- Lab 12: Worksheet
- Lab 13: Worksheet

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ELISA:

1. Record results of Widal test using + or - signs:

extremely agglutinated (+++)
very agglutinated (++)
a little agglutinated (+)
no agglutination ( – )

2. Positive results should lead to agglutination (clumping) of antibody to antigen. Your titer is the lowest dilution that had a positive result. CIRCLE YOUR TITER BELOW.

Dilution	1:20	1:40	1:80	1:160	1:320	1:640

ELISA: enzyme-linked immunosorbent assay

ELISA is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones using antibodies and color changes. ELISA is a common medical and research lab technique.

ELISA:

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