Lab 13: ELISA
- Page ID
- 24062
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ELISA:
1. Record results of Widal test using + or - signs:
- extremely agglutinated (+++)
- very agglutinated (++)
- a little agglutinated (+)
- no agglutination ( – )
2. Positive results should lead to agglutination (clumping) of antibody to antigen. Your titer is the lowest dilution that had a positive result. CIRCLE YOUR TITER BELOW.
| Dilution | 1:20 | 1:40 | 1:80 | 1:160 | 1:320 | 1:640 |
ELISA: enzyme-linked immunosorbent assay
ELISA is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones using antibodies and color changes. ELISA is a common medical and research lab technique.
