Search
- Filter Results
- Location
- Classification
- Include attachments
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.02%3A_Lab_Manual-_Introduction_to_Biotechnology/2.2.16%3A_ELISAIn the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibod...In the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibodies in their blood bind to the HIV antigen, the secondary antibody will bind to the human antibodies, and the enzyme will produce a colored product that is easy to visualize.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Book%3A_General_Microbiology_Lab_Manual_(Pakpour_and_Horgan)/Lab_13%3A_ELISAELISA: Record results of Widal test using + or - signs: a little agglutinated (+) Positive results should lead to agglutination (clumping) of antibody to antigen. Your titer is the lowest dilution tha...ELISA: Record results of Widal test using + or - signs: a little agglutinated (+) Positive results should lead to agglutination (clumping) of antibody to antigen. Your titer is the lowest dilution that had a positive result. ELISA: enzyme-linked immunosorbent assay ELISA is a plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies, and hormones using antibodies and color changes. ELISA is a common medical and research lab technique.
- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.17%3A_ELISAIn the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibod...In the ELISA conducted for this lab, the antigen (from HIV virus) is adsorbed to the surface of the plastic wells (on the 8-well strip or 96-well plate). In summary, for patients with HIV, the antibodies in their blood bind to the HIV antigen, the secondary antibody will bind to the human antibodies, and the enzyme will produce a colored product that is easy to visualize.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Fundamentals_of_Biochemistry_(Jakubowski_and_Flatt)/01%3A_Unit_I-_Structure_and_Catalysis/03%3A_Amino_Acids_Peptides_and_Proteins/3.5%3A_Extension_-_Antibodies_in_Quantitation_and_In_Vivo_DetectionThe page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen intera...The page details the fundamentals of antibodies in biochemistry, focusing on their structure, function, and the techniques used to utilize them. Key areas include understanding antibody-antigen interactions, exploring quantitation methods like ELISA, and discussing techniques like immunohistochemistry (IHC) used for detecting proteins in vivo.
