Lab 3: Worksheet

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Aug 1, 2021
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- Lab 3: Simple, Negative, and Gram Stain
- Lab 4: Acid-Fast, Spores, and Capsule Stains

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Name: ______________________________

Section: _____________________________

Student ID#:__________________________

Work in groups on these problems. You should try to answer the questions without referring to your textbook. If you get stuck, try asking another group for help.

1. Why are S. epidermidis and P. aeruginosa the same color with the simple stain? (1)

2. What is being stained in a negative stain? (1)

3. How does the charge of bacterial cells impact the staining procedures used? (1)

4. Which plate had gram-negative bacteria? What color were these bacteria and why? (2)

5. Which plate had gram-positive bacteria? What color were they and why? (2)

6. Draw the membrane layers of a gram-positive and gram-negative bacteria making sure to label the plasma membrane, peptidoglycan layers, periplasmic space, and LPS (3)

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