Genetics Lab - Synthetic Biology CURE (Reyna)
- Page ID
- 79489
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The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
- Front Matter
- 1: Inoculation
- 2: Lab Safety and Laboratory Notebook
- 3: Tips for Organizing the Lab for Large Classes
- 4: Visual Representation of the Protocol
- 5: Plasmid DNA Extraction (Mini-Prep)
- 6: Appendix III- Using the Nanodrop
- 7: DNA Double Digestion
- 8: Gel Loading and Running
- 9: Ligation of Products
- 10: Cell Transformation
- 11: Appendix I- Antibiotics and Color Coding
- 12: Appendix V- Common Agarose Gel Questions and Tips
- 13: Before you begin a semester overview
- Back Matter
Thumbnail: A diagram of a typical prokaryotic cell. (Public Domain; Mariana Ruiz Villarreal (LadyofHats) via Wikipedia).