Genetics Lab - Synthetic Biology CURE (Reyna)
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The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
Front Matter
1: Inoculation
2: Lab Safety and Laboratory Notebook
3: Tips for Organizing the Lab for Large Classes
4: Visual Representation of the Protocol
5: Plasmid DNA Extraction (Mini-Prep)
6: Appendix III- Using the Nanodrop
7: DNA Double Digestion
8: Gel Loading and Running
9: Ligation of Products
10: Cell Transformation
11: Appendix I- Antibiotics and Color Coding
12: Appendix V- Common Agarose Gel Questions and Tips
13: Before you begin a semester overview
Back Matter
Thumbnail: A diagram of a typical prokaryotic cell. (Public Domain; Mariana Ruiz Villarreal (LadyofHats) via Wikipedia).