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- https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Synthetic_Biology_Protocols/02%3A_Appendices/2.03%3A_Appendix_III-_Using_the_NanodropThe NanoDrop allows you to measure the concentration of DNA, RNA, and protein in a couple of microliters of the solution. Lift the upper arm of the NanoDrop and remove the wipe. Lift the upper arm and...The NanoDrop allows you to measure the concentration of DNA, RNA, and protein in a couple of microliters of the solution. Lift the upper arm of the NanoDrop and remove the wipe. Lift the upper arm and use the wipe to vigorously scrub both the upper and lower pedestals. Lower the upper arm of the NanoDrop and click the “Blank” button on the software. Lift the upper arm and dry the pedestal OR lift the upper arm and carefully pipet up as much of the sample as you can to retain for further use.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.03%3A_Lab_Manual-_Synthetic_Biology_Protocols/2.3.01%3A_Getting_Started-Instructor's_Guide/2.3.1.02%3A_Adding_AntibioticsAmpicillin (Amp/A): *Working concentration 100 ug/mL freezer stock 100 mg/mL (in water) Add 1 mL of 100 mg/mL ampicillin (dissolved in water) into 1 L of LB broth or agar Add 1 mL of 35 mg/mL chloramp...Ampicillin (Amp/A): *Working concentration 100 ug/mL freezer stock 100 mg/mL (in water) Add 1 mL of 100 mg/mL ampicillin (dissolved in water) into 1 L of LB broth or agar Add 1 mL of 35 mg/mL chloramphenicol (dissolved in ethanol) into 1 L of LB broth or agar Add 1 mL of 50 mg/mL kanamycin (dissolved in water) into 1 L of LB broth or agar Add 1 mL of 10 mg/mL tetracycline (dissolved in water) into 1 L LB broth or agar
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.03%3A_Lab_Manual-_Synthetic_Biology_Protocols/2.3.01%3A_Getting_Started-Instructor's_Guide/2.3.1.01%3A_Making_Media_(LB_Broth_and_Agar)Making 1 L of LB Agar: Let cool before pouring into plates (approximately 25 mL of agar in each plate) This recipe makes about 40 plates and can be adjusted as needed! Making 1 L of LB Broth: *This po...Making 1 L of LB Agar: Let cool before pouring into plates (approximately 25 mL of agar in each plate) This recipe makes about 40 plates and can be adjusted as needed! Making 1 L of LB Broth: *This powder contains 10 g tryptone, 5 g yeast extract and 10 g NaCl* Let cool before pouring into STERILE test tubes with lids (approximately 5 mL of broth per test tube) This recipe makes about 200 tubes of broth and can be adjusted as needed!
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.03%3A_Lab_Manual-_Synthetic_Biology_Protocols/2.3.03%3A_Appendices-_Equipment_Use
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.03%3A_Lab_Manual-_Synthetic_Biology_Protocols/2.3.02%3A_Student_Lab_Manual-_Protocols/2.3.2.01%3A_Visual_Representation_of_the_ProtocolCreated with biorender.com
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.03%3A_Lab_Manual-_Synthetic_Biology_Protocols/2.3.03%3A_Appendices-_Equipment_Use/2.3.3.04%3A_Appendix_IV-_Using_the_QubitThe Qubit allows you to measure the concentration of DNA, RNA, and protein in a couple of microliters of the solution. On the “Home Screen,” choose the type of assay for which you want to run the stan...The Qubit allows you to measure the concentration of DNA, RNA, and protein in a couple of microliters of the solution. On the “Home Screen,” choose the type of assay for which you want to run the standards. Upon the completion of the measurement, the result is displayed on the screen. The number displayed is the concentration of the nucleic acid in the assay tube. To read the next sample, remove the sample from the Sample Chamber, insert the next sample, and press “Read Next Sample.”
- https://bio.libretexts.org/Courses/Ouachita_Baptist_University/Genetics_Lab_-_Synthetic_Biology_CURE_(Reyna)/zz%3A_Back_Matter/10%3A_Index
- https://bio.libretexts.org/Courses/Ouachita_Baptist_University/Genetics_Lab_-_Synthetic_Biology_CURE_(Reyna)/13%3A_Before_you_begin_a_semester_overviewThe protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or B...The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions. The process of engineering the device requires iteration of standard molecular biology cloning techniques which are outlined in this lab manual, some including video protocols.
- https://bio.libretexts.org/Courses/Ouachita_Baptist_University/Genetics_Lab_-_Synthetic_Biology_CURE_(Reyna)/00%3A_Front_Matter
- https://bio.libretexts.org/Courses/Ouachita_Baptist_University/Genetics_Lab_-_Synthetic_Biology_CURE_(Reyna)The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or ...The protocols included in this lab manual are part of a Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions.
- https://bio.libretexts.org/Courses/Ouachita_Baptist_University/Genetics_Lab_-_Synthetic_Biology_CURE_(Reyna)/02%3A_Lab_Safety_and_Laboratory_NotebookNumber each page of the notebook in the upper corner with the first page of the notebook being “page 1.” The back of the first page will be “page 2” and so on. It is the responsibility of each person ...Number each page of the notebook in the upper corner with the first page of the notebook being “page 1.” The back of the first page will be “page 2” and so on. It is the responsibility of each person in the lab to know and follow basic laboratory safety rules, to understand how to safely operate equipment, understand the hazards of materials they are working with and to work to reduce potential risks.
