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1.2: Formative/Summative Questions

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    Formative Questions

    Parts of the Compound Light Microscope

    Figure \(\PageIndex{1}\): Side and front view of Olympus CX43 microscope, from user manual.

    I tried to leave a comment here and the pics of the microscope disappeared.

    Instructions: Identify & label the following parts of your microscope onto the image above, and fill-in-the blanks below.

    · Binocular head, Oculars: _______x

    · Arm

    · Revolving nosepiece

    · Objective lenses: ______x, _______x, _______x, _______x

    · Mechanical stage and X/Y knobs

    · Stage/slide clips

    · Condenser Turret __________, ___________, __________, ____________

    · Iris diaphragm and how to adjust it

    · Coarse adjustment knob & fine adjustment knob

    · Light source; illuminator

    · Base

    · Dimmer; light intensity

    · Power switch & power cord

    · Total magnification calculation =

        Specimens Viewed Under the Compound Light Microscope

    View both Paramecium and Amoeba slides under the 4x, 10x, and 40x objectives, and sketch and label each organism in the space below. 

    Circular space to sketch and label organism            Circular space to sketch and label organism 

    Summative Questions

    Proper Use of the Compound Light Microscope

    1. Describe the steps needed to focus a specimen on your slide at 100x total magnification.

    Answer: Students should know to start with the 4x objective lens. Center specimen and use the coarse adjustment knob to focus before going to 10x objective lens.

    1. A student has focused a specimen at 100x total magnification and plans to view the specimen at 400x total magnification next. Why is the coarse adjustment knob not necessary when focusing the slide at 400x total magnification? 

    Answer: Movements with the coarse adjustment knob are too "big." Smaller movements with the fine adjustment knob are needed to focus at 400x total magnification.

    1. Describe the steps necessary to store your microscope away properly after use.

    Answer:

    1. Turn off power.
    2. Remove slide.
    3. Rotate 4x objective lens into place.
    4. Lower and center stage.
    5. Wipe lenses with lens paper.
    6. Wrap cord.
    7. Cover with dust cover.
    8. Place in appropriate cabinet.
       

    Lab Safety Guidelines

    1. State the location of the classroom emergency phone, emergency exits, and fire extinguisher.

    Answer: variable. Identify before class starts.

    1. What are the proper safety protocols to complete at the beginning and end of each lab?

    Answer: Variable. Protocols include washing hands before and after lab. Wipe down desk/counter surfaces before and after lab.

    This page titled 1.2: Formative/Summative Questions is shared under a CC BY 4.0 license and was authored, remixed, and/or curated by Darcy Ernst, May Chen, Katie Foltz, and Bridget Greuel (Open Educational Resource Initiative at Evergreen Valley College) .