- Explain how the bacterial cells, normally not competent, were made competent during the procedure.
- Differentiate between the purposes of each of the 4 plates used in the exercise.
- Contrast the uses of the antibiotic and arabinose in the exercise.
- Why did the P- bacteria grow on the LB agar without ampicillin and not in the Petri dish with the LB agar with the ampicillin?
- For the P+ plates, why did colonies "glow" on one plate and not the other?
- How is the use of Arabinose a means of gene regulation?
- E. coli cells can double about every 20 minutes with the proper environmental conditions. How long would it take a single transformed cell to become 100 million cells, all with resistance to ampicillin?
Contributors and Attributions
Kelly C. Burke (College of the Canyons)