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Biology LibreTexts

16.1: Learning Objectives

  • Page ID
    40253
  • Learning Objectives

    After this lab you should be able to:

    1. Define the vocabulary related to transformation, recombinant plasmid, selectable marker, vector, and competent cell, genetic engineering, gene regulation, etc.
    2. Relate the mechanism of genetic recombination, specifically transformation, to the problem of antibiotic resistant bacterial infections.
    3. Describe how gene regulation can be affected by a bacterium’s environment.
    4. Outline the process of transformation in bacteria.
    5. Evaluate the outcomes of the bacterial transformation activity.
    clipboard_e24bee5b16b737109cce4560c58a8bc1f.png
    Figure \(\PageIndex{1}\): Source: http://www.cdc.gov/drugresistance/about.html

    Antibiotic resistance is a critical problem in healthcare today. How do bacteria become resistant to antibiotics? In this lab we will focus on #4 from the CDC infographic above. Some bacteria can transfer genes from one bacterium to another in a process called “conjugation” or may pick up genes from the surrounding environment (from dead bacteria), via “transformation”. These genes, often on a plasmid, may code for antibiotic resistance mechanisms.

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