After this lab you should be able to:
- Define the vocabulary related to transformation, recombinant plasmid, selectable marker, vector, and competent cell, genetic engineering, gene regulation, etc.
- Relate the mechanism of genetic recombination, specifically transformation, to the problem of antibiotic resistant bacterial infections.
- Describe how gene regulation can be affected by a bacterium’s environment.
- Outline the process of transformation in bacteria.
- Evaluate the outcomes of the bacterial transformation activity.
Antibiotic resistance is a critical problem in healthcare today. How do bacteria become resistant to antibiotics? In this lab we will focus on #4 from the CDC infographic above. Some bacteria can transfer genes from one bacterium to another in a process called “conjugation” or may pick up genes from the surrounding environment (from dead bacteria), via “transformation”. These genes, often on a plasmid, may code for antibiotic resistance mechanisms.
Contributors and Attributions
Kelly C. Burke (College of the Canyons)