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Biology LibreTexts

13.4: Materials and Procedures

  • Page ID
    40235
  • Materials

    2 Groups per Table:

    • Organisms-
      • Staphylococcus saprophyticus
      • Escherichia coli
      • Lactococcus lactis
    • 1 plate of each Sodium Chloride (\(\ce{NaCl}\)) concentration:
    • TSA with 2.0% \(\ce{NaCl}\)
    • TSA with 5.0% \(\ce{NaCl}\)
    • TSA with 10.0%\(\ce{NaCl}\)

    Procedures

    Inoculate each plate with the following organisms: Staphylococcus saprophyticus, Escherichia coli, Lactococcus lactis

    1. Use a single streak on ⅓ of the plate for each organism; make sure to label each streak line with the correct organism:

    Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 2.0% \(\ce{NaCl}\)

    Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 5.0% \(\ce{NaCl}\)

    Staphylococcus saprophyticus, E. coli, Lactococcus lactis → TSA with 10.0% \(\ce{NaCl}\)

    1. Invert and incubate.

    Results

    1. After incubation assess the growth of each bacteria on each plate.
    2. Fill out the chart below:

    Enter your ratings for each bacterium/salinity and give the salinity classification for bacterium.

    Organism

    2.0% \(\ce{NaCl}\)

    5.0% \(\ce{NaCl}\)

    10.0% \(\ce{NaCl}\)l

    Optimum salinity

    Salinity Classification

    Contributors and Attributions

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