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2.12: Glycoproteins

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    Glycoproteins have carbohydrate attached to them — a process called glycosylation. The attachment is a covalent linkage to:

    • the hydroxyl (-OH) group of the R group of serine or threonine - called "O-linked" in both cases or to
    • the amino group (-NH2) in the R group of asparagine - called "N-linked"

    The carbohydrate consists of short, usually branched, chains of

    • plain sugars (e.g., glucose, galactose)
    • amino sugars (sugars with an amino group, e.g., N-acetylglucosamine), and
    • acidic sugars (sugars with a carboxyl group, e.g., sialic acid)

    Sugars are very hydrophilic thanks to their many -OH groups. Their presence

    • makes glycoproteins far more hydrophilic than they would be otherwise and
    • are often essential for the proper folding of the protein into its tertiary structure

    Most of the proteins exposed to the watery surroundings at the surface of cells are glycoproteins.

    altThis image shows the primary structure of glycophorin A, a glycoprotein that spans the plasma membrane ("Lipid bilayer") of human red blood cells. Each RBC has some 500,000 copies of the molecule embedded in its plasma membrane.

    • Fifteen carbohydrate chains are "O-linked" to serine (Ser) and threonine (Thr) residues
    • One carbohydrate chain is "N-linked" to the asparagine (Asn) at position 26

    Two polymorphic versions of glycophorin A, which differ only at residues 1 and 5, occur in humans. These give rise to the MN blood groups

    • The M allele encodes Ser at position 1 (Ser-1) and Gly at position 5 (Gly-5)
    • The N allele encodes Leu-1 and Glu-5

    Genotype to Phenotype

    Individuals who inherit two N alleles (are homozygous) have blood group N.

    • Individuals who are homozygous for the M allele have blood group M.
    • Heterozygous individuals produce both proteins and have blood group MN.

    Glycophorin A is the most important attachment site by which the parasite Plasmodium falciparum invades human red blood cells.

    This page titled 2.12: Glycoproteins is shared under a CC BY 3.0 license and was authored, remixed, and/or curated by John W. Kimball via source content that was edited to the style and standards of the LibreTexts platform; a detailed edit history is available upon request.

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