3.1.7: DNA Ligation Supplies
- Page ID
- 67614
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DNA Ligation:
|
Materials |
Amount Needed (Per Reaction) |
|
Distilled Water |
5 uL (up to 10 uL) |
|
10X DNA Ligation Buffer (Cat#: B0202S) |
1 uL |
|
Linearized Plasmid Backbone (Amp,Chlor,Kan,Tet) |
1 uL |
|
Double Digested DNA insert fragments |
1 uL |
|
T4 DNA ligase (Cat#: M0202S) |
1 uL |
|
Ice Bucket |
1 |
|
Microcentrifuge Tube |
1 |
|
Micropipette and Tips |
1-10 uL |
|
Heat Block (80C) |
1 (used by class) |