3.1.6: Gel Loading/Running Supplies
- Page ID
- 67613
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Gel Loading and Running:
|
Materials |
Amount Needed (Per Reaction) |
|
Agarose |
Varies depending on gel percentage |
|
1x TBE |
Approximately 165 mL (to make gel and for gel rig) |
|
SybrGreen, SybrSafe, or GelRed |
2-3 uL |
|
Digested DNA |
5 uL |
|
DNA Ladder (Quick-Load Ladder Cat#: N0550S) |
5 uL |
|
6x DNA Loading Dye (Cat#: B7024S) |
1 uL |
|
Microwave |
1 (used by class) |
|
Flask |
1 (at least 50 mL in size) |
|
Micropipette and Tips |
1-10 uL |
|
Gel Rig (MiniOne; Cat#s: M1000, M1002, M1010, M1012) |
1 (with all necessary parts included with the rig) |
|
Weigh Tray |
1 |
|
Scale |
1 (used by class) |