3.1.8: Transformation Supplies
- Page ID
- 67615
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Transformation:
|
Materials |
Amount Needed (Per Reaction) |
|
Competent Bacteria Cells (JM109= Cat#: T3003) |
25-50 uL |
|
Ligation Mixture |
1-5 uL |
|
LB Agar with Antibiotics (either Kan, Chlor, Amp, or Tet) |
1 plate (depending on backbone) |
|
SOC Media (Cat#: 15544034) |
200 uL |
|
Sterile Glass Beads |
Approximately 4-8 beads |
|
Ice Bucket |
1 |
|
Microcentrifuge Tube |
1 |
|
Shaking Incubator (37C) |
1 (used by class) |
|
Plate Warmer (37C) (Can be the same as the Shaking Incubator) |
1 (used by class) |
|
Incubator |
1 (used by class) |