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Lab Manual: Synthetic Biology Protocols

  • Page ID
    • Nathan Reyna, Ruth Plymale, & Kristen Johnson
    • Ouachita Babtist University & University of New Hampshire
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    The protocols and instructor guide included in this lab manual are part of a semester-long Synthetic Biology undergraduate research course (CURE) designed by the authors. The lab presented in this manual is designed for a Genetics or Biotechnology class and has been used at both two-year and four-year institutions. Students are tasked with designing and then engineering a biological device in a bacterium (E. coli) using standardized DNA parts called Bio-Bricks from the iGEM ( registry. The process of engineering the device requires iteration of standard molecular biology cloning techniques which are outlined in the lab manual, some including video protocols. Additionally, provided are recipes for reagents, a supply/resources list, and instructor recommendations. A series of faculty training videos were created to further help with the implementation of the SynBio CURE.

    • Authors: Nathan Reyna (project PI; Ouachita Baptist University, Arkadelphia, AR), Ruth Plymale (Ouachita Baptist University, Arkadelphia, AR), Kristen Johnson (University of New Hampshire, Manchester, NH)
    • Protocol editing and appendix contributors: Jonathan Hoying, Sadie Vezina (University of New Hampshire, Manchester, NH), Jaime Sabel (University of Memphis, Memphis, TN)
    • Demonstration video contributions: Jade Dorman, Lauran McCann, Gracen Hambrick (Ouachita Baptist University, Arkadelphia, AR)

    Thumbnail: CRISPR Cas9 system (CC BY-SA 4.0; marius walter via Wikipedia)

    This page titled Lab Manual: Synthetic Biology Protocols is shared under a not declared license and was authored, remixed, and/or curated by Nathan Reyna, Ruth Plymale, & Kristen Johnson.