Encyclopedia of Biological Methods (Mattaini)
- Page ID
- 135755
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)The Encyclopedia of Biological Methods (EOBM) helps solve a problem faced by many biology faculty: it is good practice to use primary research literature in our classes, but not all students have the same background in all the different methods, both historical and modern, used in journal articles. The EOBM is written to introduce biological methods to any student with a basic understanding of introductory-level college biology. It introduces information at the correct level of technicality, can be more trusted and directed than the results of a Google search performed by an undergraduate, and can directly address common undergraduate misconceptions. Furthermore, most entries have an "interpretation" section that explains the results of a published data figure using the method, giving students real-life practice with data interpretation.
This is a long-term work in progress, and I welcome entries from other faculty. There is also the possibility of making student-written, faculty-reviewed entries into projects for biology courses, or opportunities for graduate students and postdocs. To discuss, please email me. Email: Katie Mattaini.
Front Matter
1: Bacterial Transformation
2: Bradford Assay
3: BrdU Assay
4: Cell Migration Assay
5: Chromatin Immunoprecipitation
6: Coimmunoprecipitation
7: Column Chromatography
8: Cox Proportional Hazards Analysis
9: CRISPR
10: DNA Microarray
11: DNase Footprinting
12: Electrophoretic Mobility Shift Assay
13: Flow Cytometry
14: Gel Electrophoresis
15: GFP Fusion Proteins
16: Immunofluorescence Microscopy
17: Immunohistochemistry
18: In Situ Hybridization
19: LC-MS
20: Molecular Cloning
21: Northern Blot and Southern Blot
22: ONPG Assay
23: PCR
24: pET Expression System
25: QIBC
26: Quantitative Real Time PCR
27: Reverse Transcription PCR
28: RNA Interference
29: Sanger Sequencing
30: Site-directed Mutagenesis
31: Site-specific Recombination
32: Soft Agar Colony Formation Assay
33: TUNEL Assay
34: Transwell Migration and Invasion Assay
35: Ultracentrifugation
36: Western Blot
Back Matter


