Encyclopedia of Biological Methods (Mattaini)
- Page ID
- 135755
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)The Encyclopedia of Biological Methods (EOBM) helps solve a problem faced by many biology faculty: it is good practice to use primary research literature in our classes, but not all students have the same background in all the different methods, both historical and modern, used in journal articles. The EOBM is written to introduce biological methods to any student with a basic understanding of introductory-level college biology. It introduces information at the correct level of technicality, can be more trusted and directed than the results of a Google search performed by an undergraduate, and can directly address common undergraduate misconceptions. Furthermore, most entries have an "interpretation" section that explains the results of a published data figure using the method, giving students real-life practice with data interpretation.
This is a long-term work in progress, and I welcome entries from other faculty. There is also the possibility of making student-written, faculty-reviewed entries into projects for biology courses, or opportunities for graduate students and postdocs. To discuss, please email me. Email: Katie Mattaini.
Front Matter
1: Bacterial Transformation
2: Bradford Assay
3: BrdU Assay
4: Cell Migration Assay
5: Coimmunoprecipitation
6: Column Chromatography
7: Cox Proportional Hazards Analysis
8: CRISPR
9: DNA Microarray
10: DNase Footprinting
11: Electrophoretic Mobility Shift Assay
12: Flow Cytometry
13: Gel Electrophoresis
14: GFP Fusion Proteins
15: Immunofluorescence Microscopy
16: Immunohistochemistry
17: In Situ Hybridization
18: LC-MS
19: Molecular Cloning
20: Northern Blot and Southern Blot
21: ONPG Assay
22: PCR
23: pET Expression System
24: QIBC
25: Quantitative Real Time PCR
26: Reverse Transcription PCR
27: RNA Interference
28: Sanger Sequencing
29: Site-directed Mutagenesis
30: Site-specific Recombination
31: Soft Agar Colony Formation Assay
32: TUNEL Assay
33: Transwell Migration and Invasion Assay
34: Ultracentrifugation
35: Western Blot
Back Matter


