Encyclopedia of Biological Methods (Mattaini)
- Page ID
- 135755
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)This book is undergoing significant updates in Fall semester 2024. Please excuse its appearance during this time!
The Encyclopedia of Biological Methods (EOBM) helps solve a problem faced by many biology faculty: it is good practice to use primary research literature in our classes, but not all students have the same background in all the different methods, both historical and modern, used in journal articles. The EOBM is written to introduce biological methods to any student with a basic understanding of introductory-level college biology. It introduces information at the correct level of technicality, can be more trusted and directed than the results of a Google search performed by an undergraduate, and can directly address common undergraduate misconceptions. Furthermore, most entries have an "interpretation" section that explains the results of a published data figure using the method, giving students real-life practice with data interpretation.
This is a long-term work in progress, and I welcome entries from other faculty. There is also the possibility of making student-written, faculty-reviewed entries into projects for biology courses, or opportunities for graduate students and postdocs. To discuss, please email me. Email: Katie Mattaini.
- Front Matter
- 1: Bacterial Transformation
- 2: Bradford Assay
- 3: BrdU assay
- 4: Cell migration assay
- 5: Coimmunoprecipitation
- 6: Column chromatography
- 7: Cox proportional hazards analysis
- 8: CRISPR
- 9: DNA Microarray
- 10: Flow Cytometry
- 11: Gel electrophoresis
- 12: GFP fusion proteins
- 13: Immunofluorescence microscopy
- 14: Immunohistochemistry
- 15: In situ hybridization
- 16: LC-MS
- 17: Molecular Cloning
- 18: Northern blot and Southern blot
- 19: ONPG Assay
- 20: PCR
- 21: pET expression system
- 22: QIBC
- 23: Quantitative real time PCR
- 24: Reverse transcription PCR
- 25: RNA interference
- 26: Site-directed mutagenesis
- 27: Site-specific recombination
- 28: Soft agar colony formation assay
- 29: TUNEL assay
- 30: Transwell migration and invasion assay
- 31: Ultracentrifugation
- 32: Western blot
- 33: Entry template
- Back Matter