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- https://bio.libretexts.org/Bookshelves/Biotechnology/Bio-OER_(CUNY)/16%3A_Bioinformatics/16.03%3A_In_Silico_RestrictionRestriction enzymes act as molecular scissors. The ones we use in Molecular Biology are those that cut within known sequences that occur often enough, yet rare enough to cut our DNA into analyzable fr...Restriction enzymes act as molecular scissors. The ones we use in Molecular Biology are those that cut within known sequences that occur often enough, yet rare enough to cut our DNA into analyzable fragments. Molecular Biologists often use 6-cutters. This means that the site of digestion is “restricted” to a recognition sequence of 6 nucleotides. These nucleotides are usually palindromic as discussed before.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/09%3A_Regulation_of_Gene_Expression/9.10%3A_PalindromesThis page explains palindromes, which read the same forwards and backwards, and highlights their significance in DNA. It discusses how palindromes serve as target sequences for restriction enzymes and...This page explains palindromes, which read the same forwards and backwards, and highlights their significance in DNA. It discusses how palindromes serve as target sequences for restriction enzymes and the role of inverted repeats in transcription factor binding. Additionally, it notes the presence of inverted repeats in transposons, retroviral genes, and the human Y chromosome, where they may improve gene repair through homologous recombination.
- https://bio.libretexts.org/Bookshelves/Genetics/Genetics_Agriculture_and_Biotechnology_(Suza_and_Lee)/01%3A_Chapters/1.11%3A_Recombinant_DNA_TechnologyRecombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating ...Recombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating genes and transform them into cells of plant and animals. To arrive at this several tools of molecular biology are used including, DNA isolation and analysis, molecular cloning, quantification of gene expression, and many others.
- https://bio.libretexts.org/Courses/City_College_of_San_Francisco/Introduction_to_Genetics/12%3A_Techniques_of_Molecular_Genetics/12.04%3A_Cutting_and_Pasting_DNA-_Restriction_Digests_and_DNA_LigationMany bacteria have enzymes that recognize specific DNA sequences and then cut the double stranded DNA helix at this sequence. These enzymes are called site-specific restriction endonucleases, or more ...Many bacteria have enzymes that recognize specific DNA sequences and then cut the double stranded DNA helix at this sequence. These enzymes are called site-specific restriction endonucleases, or more simply “restriction enzymes”, and they naturally function as part of bacterial defenses against viruses and other sources of foreign DNA. To cut DNA at known locations, researchers use restriction enzymes from various bacterial species, and which can be purchased from various commercial sources.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/05%3A_DNAThis page discusses bacterial genetic recombination methods, including transformation, conjugation, and transduction. It highlights key experiments on DNA's role as genetic material, notable discoveri...This page discusses bacterial genetic recombination methods, including transformation, conjugation, and transduction. It highlights key experiments on DNA's role as genetic material, notable discoveries in its double helix structure, and semi-conservative replication. Additionally, it mentions the importance of restriction enzymes for DNA manipulation and recent advancements in sequencing techniques that enhance genome analysis.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.04%3A_Genetics_Agriculture_and_Biotechnology_(Suza_and_Lee)/2.4.11%3A_11_-_Recombinant_DNA_TechnologyRecombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating ...Recombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating genes and transform them into cells of plant and animals. To arrive at this several tools of molecular biology are used including, DNA isolation and analysis, molecular cloning, quantification of gene expression, and many others.
- https://bio.libretexts.org/Bookshelves/Biochemistry/Book%3A_Biochemistry_Free_For_All_(Ahern_Rajagopal_and_Tan)/08%3A_Basic_Techniques/8.06%3A_Isolating_GenesMethods to isolate genes were not available till the 1970s, when the discovery of restriction enzymes and the invention of molecular cloning provided, for the first time, ways to obtain large quantiti...Methods to isolate genes were not available till the 1970s, when the discovery of restriction enzymes and the invention of molecular cloning provided, for the first time, ways to obtain large quantities of specific DNA fragments, for study. Although, for purposes of obtaining large amounts of a specific DNA fragment, molecular cloning has been largely replaced by direct amplification using the polymerase chain reaction described later, cloned DNAs are still very useful for a variety of reasons.
- https://bio.libretexts.org/Bookshelves/Microbiology/Microbiology_(Boundless)/17%3A_Industrial_Microbiology/17.01%3A_Industrial_Microbiology/17.1B%3A_Molecular_Products_from_MicrobesThe isolation of molecular products from microbes is considered to be a key component of molecular biology research.
- https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/11%3A_Genomics/11.07%3A_Restriction_Fragment_Length_PolymorphismsThis page discusses the use of restriction enzymes and probes in DNA analysis, highlighting the significance of RFLPs for gene screening and forensic applications, despite challenges like mutation var...This page discusses the use of restriction enzymes and probes in DNA analysis, highlighting the significance of RFLPs for gene screening and forensic applications, despite challenges like mutation variability. It notes the evolution of DNA profiling in criminal investigations, emphasizing that using multiple probes and STRs can significantly reduce misidentification probabilities. Since 1999, U.S.
- https://bio.libretexts.org/Courses/San_Diego_State_University/BT@SDSU/02%3A_Resources_and_Reading_Materials/2.01%3A_Bio-OER_(CUNY)/2.1.16%3A_Bioinformatics/2.1.16.03%3A_In_Silico_RestrictionRestriction enzymes act as molecular scissors. The ones we use in Molecular Biology are those that cut within known sequences that occur often enough, yet rare enough to cut our DNA into analyzable fr...Restriction enzymes act as molecular scissors. The ones we use in Molecular Biology are those that cut within known sequences that occur often enough, yet rare enough to cut our DNA into analyzable fragments. Molecular Biologists often use 6-cutters. This means that the site of digestion is “restricted” to a recognition sequence of 6 nucleotides. These nucleotides are usually palindromic as discussed before.
- https://bio.libretexts.org/Courses/Ohio_State_University/Ohio_State_University_SP22%3A_Molecular_Genetics_4606_(Chamberlin)/12%3A_Evaluating_Recombinant_DNA/12.03%3A_Recombinant_DNA_TechnologyRecombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating ...Recombinant DNA (rDNA) technology has resulted in breakthroughs in crop and animal biotechnology. The power of rDNA technology comes from our ability to study and modify gene function by manipulating genes and transform them into cells of plant and animals. To arrive at this several tools of molecular biology are used including, DNA isolation and analysis, molecular cloning, quantification of gene expression, and many others.
