Amylase is an exoenzyme (an enzyme released from the bacteria into its surroundings) that breaks starch by cleaving large starch molecules into monosaccharide and disaccharide units that can then enter the cell and be metabolized.
- Obtain a deep of starch agar, melt it and prepare a Petri plate.
- After the starch agar has solidified, using the inoculation loop, make one streak off center of the plate with your assigned bacterium.
- Incubate the plate upside down for at least 48 hours.
- After the incubation period, flood the plate with iodine.
Iodine reacts with starch resulting in a blue/blue black color. Therefore, any area containing starch will turn dark blue/black/purple. Areas free of starch will remain clear.
In a positive reaction, bacteria producing amylase will have a clear halo around them.