10.2: Procedures

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Procedure A. The Fungi

Observe the following slides under the microscope. Use the space provided to draw diagrams of the slides to help you remember them.

1. Rhizopus stolonifor- zygospore

2. Rhizopus stolonifor- sporangiospore

3. Penicillium notatum- conidiospore

4. Aspergillus niger- conidiospore

5. Candida albicans

6. Saccharomyces cereviseae (budding yeast)

7. Assorted live molds (set up on demo scopes)

Screenshot (151).png

Procedure B. The Protozoa

Observe the slides listed above. Draw diagrams to help you remember the organisms. Then use the reference materials available in the lab to fill in the information below for each organism in Table 10.2.1 (your instructor may assign you to research a particular species or group).

Screenshot (151).png

Table 10.2.1: Protazoa

Organism (species name)	Taxonomic grouping (based on mode of locomotion)	Disease	How transmitted	Part of body affected	Other info

Procedure C. The Helminthes

Observe the following slides and preserved specimens. Use the spaces provided to draw diagrams that will help you remember them. Then use the sources of information provided to fill in Table 10.2.2 (your instructor may assign you to research a particular species or group of species)

1. Enterobius vermicularis

2. Necator americanus

3. Schistosoma mansoni

4. Fasciola hepatica

5. Trichinella spiralis

6. Wuchereria bancrofti

7. Taenia solium

8. Preserved samples of tapeworms

9. Preserved samples of Ascaris lumbricoides

Screenshot (151).png

Table 10.2.2: Helminthes

Species name	Common name	Taxonomic grouping(s)	How transmitted	Part of body affected	Other information

Procedure D. Forehead cultures

In this lab you will culture bacterial inhabitants of a human forehead, and in Lab 12 you will use the Biolog bacterial identification system to identify the isolate.

Per pair of students: 1 TSA plate

1. Each student will swab their forehead with a sterile cotton swab, and then swab the TSA plate. A composite culture will be used so a specific bacterial species cannot be linked to a particular student.

2. Plates will be incubated until the next lab period.