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- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/12%3A_Clinical_Microbiology_Part_II-_Immunology_and_the_Biolog_System/12.04%3A_Review_QuestionsWhy are the washing steps necessary in the ELISA test? What is the purpose of the enzyme conjugated to the secondary antibody in the ELISA test? Why is it important to include a negative control well ...Why are the washing steps necessary in the ELISA test? What is the purpose of the enzyme conjugated to the secondary antibody in the ELISA test? Why is it important to include a negative control well on the Biolog plate? What does it mean if an environmental sample does not match 100% to a species in the database? What possible error do you think could occur by inoculating too much bacteria into the Biolog wells?
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)The exercises in this laboratory manual are designed to engage students in hand-on activities that reinforce their understanding of the microbial world. Topics covered include: staining and microscopy...The exercises in this laboratory manual are designed to engage students in hand-on activities that reinforce their understanding of the microbial world. Topics covered include: staining and microscopy, metabolic testing, physical and chemical control of microorganisms, and immunology. The target audience is primarily students preparing for a career in the health sciences, however many of the topics would be appropriate for a general microbiology course as well.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/16%3A_Introduction_to_MicropipettingThe volume is set by turning the knob at the top of the pipettor until the correct volume is reached. Open the box of tips and insert the end of the pipettor into the end of a pipet tip. If you depres...The volume is set by turning the knob at the top of the pipettor until the correct volume is reached. Open the box of tips and insert the end of the pipettor into the end of a pipet tip. If you depress the liquid after placing the tip in the liquid you will be blowing air into the liquid! If you tilt it too much the liquid in the tip will make contact with the pipettor and will no longer be sterile (this could also damage the pipettor).
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/06%3A__Dichotomous_Keys
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/02%3A_Introduction_to_Aseptic_Techniques_and_Growth_Media/2.03%3A_ResultsUnknown A: Describe growth characteristics on MSA and make a sketch in the circle below. Unknown B: Describe growth characteristics on EMB and make a sketch in the circle below. Record the results of ...Unknown A: Describe growth characteristics on MSA and make a sketch in the circle below. Unknown B: Describe growth characteristics on EMB and make a sketch in the circle below. Record the results of your own environmental sample and the other sample at your bench in the two tables below. Was there evidence for organisms other than bacteria? (Ask your instructor.) Your instructor may ask you to save some of your environmental samples for further analysis.
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/05%3A_Metabolic_Activities_of_Bacteria/5.03%3A_ResultsIt is important to obtain results all of the organisms in the table, as you will need this information to identify your unknown organisms for your midterm practical. You can compare your inoculated tu...It is important to obtain results all of the organisms in the table, as you will need this information to identify your unknown organisms for your midterm practical. You can compare your inoculated tubes with the negative controls in the front of the class at the instructor’s table. Add enough Gram’s iodine to the plate to ensure that the entire areas of growth and the agar surrounding it are covered (you should also add iodine to the negative control area).
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/08%3A__Chemical_Control_of_Microbial_Growth_-_Disinfectants_and_Antibiotics/8.03%3A_ResultsUse the spaces below to sketch the appearance of your own TSA plate and your lab partner’s plate. Do you see any small colonies within the zones of inhibition on any of your plates? Use the charts pro...Use the spaces below to sketch the appearance of your own TSA plate and your lab partner’s plate. Do you see any small colonies within the zones of inhibition on any of your plates? Use the charts provided by your instructor to look up the interpretation for each of the zones you measured. Measure the zones of inhibition and record your observations in the table below. Do you notice any differences in the ability of the Streptomyces strains to inhibit gram positive and gram negative bacteria?
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/09%3A_Microbiology_of_Milk_and_Food
- https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/02%3A_Cultivation_of_Microbes/2.06%3A_Review_QuestionsWhat general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) If the bacteria ferment lactose, what would you expect to see? What mediu...What general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) If the bacteria ferment lactose, what would you expect to see? What medium would you use (TSA, EMB, MSA) if you wanted to determine if a Staphylococcus isolate could ferment mannitol? If you were testing water for the presence of fecal coliforms, what sort of medium would you use: TSA, EMB agar or MSA agar?
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/04%3A_Acid_fast_and_Endospore_Staining/4.02%3A_ProceduresPrepare a slide with Mycobacterium smegmatis on one side and Micrococcus luteus on the other side. (Alternatively, both bacteria may be mixed into one smear). Acid-fast organisms retain the primary st...Prepare a slide with Mycobacterium smegmatis on one side and Micrococcus luteus on the other side. (Alternatively, both bacteria may be mixed into one smear). Acid-fast organisms retain the primary stain and will appear bright red: non acid-fast organisms are decolorized with acid-alcohol and pick up the methylene blue counterstain. Prepare a smear with Bacillus subtilis or Clostridium sporogenes on one side of the slide, any other bacteria on the other side
- https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Laboratory_Exercises_in_Microbiology_(McLaughlin_and_Petersen)/06%3A__Dichotomous_Keys/6.01%3A_Introduction_to_Dichotomous_KeysIn this exercise, you will be using the metabolic tests you have learned about in Lab 5 to design a dichotomous key that will allow you determine which tests can be used to distinguish the 10 bacteria...In this exercise, you will be using the metabolic tests you have learned about in Lab 5 to design a dichotomous key that will allow you determine which tests can be used to distinguish the 10 bacterial species we are using in the lab from each other.
