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20.5: Post-lab Questions

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    1. Does a negative endospore stain eliminate the presence of endospore forming bacteria in a specimen? Explain.
    2. How might age of the culture affect the results of the endospore stain?
    3. What is the purpose of heating the cells during the primary stain, Malachite Green, step?
    4. Why do the spores not stain pink after the addition of Safranin?

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