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Biology LibreTexts

14.4: Materials and Procedures

  • Page ID
  • Materials

    • 3 FTM tubes
    • 2 TSA plates
    • GasPak jar, sachets, indicator
    • Bacterial cultures:
      • Staphylococcus saprophyticus (Use student stock organism)
      • Pseudomonas fluorescens (Use student stock organism)
      • Clostridium sporogenes (Use slant on front table, one per table only)


    1. Quickly streak each of the 3 organisms onto 2 TSA plates:
    1. Immediately place 1 plate in the anaerobe jar. Incubate the other plate aerobically in your class incubator.
    2. Inoculate each organism into a separate Fluid Thioglycolate Medium(FTM):

    Staphylococcus saprophyticus, Pseudomonas fluorescens, Clostridium sporogenes

    1. Incubate FTM tubes in your class incubator.


    1. GasPak: Compare the GasPak plate to the plate incubated aerobically and fill in the following chart.


    GasPak growth (+/-)

    Aerobic growth (+/-)

    Oxygen requirement type

    1. FTM: Draw your FTM tubes and label each with the appropriate oxygen requirement type

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