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9.4: Materials and Procedures

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    Over time you will build stocks and evaluate growth patterns of the following: Organisms will be posted on the days you are to subculture them.

    1 TSA Plate, 1 TSA Slant, 1 TSA Broth for each of the following:

    • Staphylococcus saprophyticus
    • Staphylococcus epidermidis
    • Lactococcus lactis
    • Micrococcus luteus
    • Sarcina aurantiaca
    • Sporosarcina ureae
    • Moraxella catarrhalis
    • Mycobacterium smegmatis
    • Corynebacterium pseudodiphtheriticum
    • Bacillus cereus
    • Alcaligenes viscolactis
    • Escherichia coli
    • Citrobacter freundii
    • Serratia marcescens
    • Pseudomonas fluorescens
    • Enterobacter aerogenes


    For each set of organisms posted:

    1. Label all plates, and create labels on tape and label all of the tubes.
    2. Do an isolation streak on a TSA plate
    3. Inoculate a TSA slant
    4. Inoculate a TSA broth
    5. Incubate all (NO parafilm in the incubator)
    6. After incubation evaluate each culture for presence of growth, its growth characteristics, and absence of contamination. If any cultures are contaminated inform and show them to your instructor as you may need to re-culture.
    7. After evaluating your new stocks, parafilm the plates and store the cultures in the student refrigerator.


    As you subculture and evaluate your new stock cultures fill in the following chart:

    (Write out the entire name of the bacterium and remember to use the correct binomial nomenclature format!)

    Name of Bacterium

    Plate Characteristics

    Slant Characteristics

    Broth Characteristics

    Pure Cultures?

    Contributors and Attributions

    This page titled 9.4: Materials and Procedures is shared under a CC BY license and was authored, remixed, and/or curated by Kelly C. Burke.

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